Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors
| Autor: | Marie Christine Kiefer-Meyer, Rim Jaber, Azeddine Driouich, Jérôme Leprince, Isabelle Gattin, Catherine Santaella, Barbara Plancot, Marie-Laure Follet-Gueye, Maïté Vicré-Gibouin, Céline Souc |
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| Přispěvatelé: | Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Neuroendocrinologie cellulaire et moléculaire, Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Différenciation et communication neuronale et neuroendocrine (DC2N), École supérieure d'ingénieurs et de techniciens pour l'agriculture (ESITPA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Terre de Lin - Société Coopérative Agricole (Terre De Lin) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Time Factors Physiology [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Arabidopsis MESH: Plant Roots Plant Science [CHIM.THER]Chemical Sciences/Medicinal Chemistry 01 natural sciences Plant Roots MESH: Receptors Pattern Recognition chemistry.chemical_compound Epitopes Cell Wall Gene Expression Regulation Plant Flax Border cells [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Arabidopsis thaliana MESH: Arabidopsis Glucans ComputingMilieux_MISCELLANEOUS Plant Proteins 2. Zero hunger 0303 health sciences biology MESH: Plant Proteins MESH: Peptidoglycan food and beverages MESH: Reactive Oxygen Species Cell biology MESH: Cell Survival Receptors Pattern Recognition MESH: Epitopes Cell Survival MESH: Glycoproteins Peptidoglycan Article Cell wall 03 medical and health sciences MESH: Cell Wall Plant Cells MESH: Glucans Botany Genetics MESH: Gene Expression Regulation Plant Extensin MESH: Flax 030304 developmental biology Glycoproteins Innate immune system fungi Callose MESH: Time Factors biology.organism_classification Plant cell MESH: Plant Cells chemistry biology.protein MESH: Biomarkers [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology Reactive Oxygen Species Biomarkers 010606 plant biology & botany Flagellin MESH: Flagellin |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2013, 163 (4), pp.1584-1597. ⟨10.1104/pp.113.222356⟩ Plant Physiology, 2013, 163 (4), pp.1584-1597. ⟨10.1104/pp.113.222356⟩ |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.113.222356⟩ |
| Popis: | Plant pathogens including fungi and bacteria cause many of the most serious crop diseases. The plant innate immune response is triggered upon recognition of microbe-associated molecular patterns (MAMPs) such as flagellin22 and peptidoglycan. To date, very little is known of MAMP-mediated responses in roots. Root border cells are cells that originate from root caps and are released individually into the rhizosphere. Root tips of Arabidopsis (Arabidopsis thaliana) and flax (Linum usitatissimum) release cells known as “border-like cells.” Whereas root border cells of pea (Pisum sativum) are clearly involved in defense against fungal pathogens, the function of border-like cells remains to be established. In this study, we have investigated the responses of root border-like cells of Arabidopsis and flax to flagellin22 and peptidoglycan. We found that both MAMPs triggered a rapid oxidative burst in root border-like cells of both species. The production of reactive oxygen species was accompanied by modifications in the cell wall distribution of extensin epitopes. Extensins are hydroxyproline-rich glycoproteins that can be cross linked by hydrogen peroxide to enhance the mechanical strength of the cell wall. In addition, both MAMPs also caused deposition of callose, a well-known marker of MAMP-elicited defense. Furthermore, flagellin22 induced the overexpression of genes involved in the plant immune response in root border-like cells of Arabidopsis. Our findings demonstrate that root border-like cells of flax and Arabidopsis are able to perceive an elicitation and activate defense responses. We also show that cell wall extensin is involved in the innate immunity response of root border-like cells. |
| Databáze: | OpenAIRE |
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