IGF-1R mRNA expression is increased in obese children
| Autor: | Mariana Teresa Sarti de Paula, Patricia Atique, Hugo Tourinho Filho, Carlos Eduardo Martinelli, Raphael Del Roio Liberatori, Rafaela Cristina Ricco, Rodrigo José Custódio, Rubens Garcia Ricco, Mariangela Carletti Queluz |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Percentile Endocrinology Diabetes and Metabolism Mrna expression medicine.medical_treatment 030209 endocrinology & metabolism Childhood obesity Body Mass Index Receptor IGF Type 1 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Obese group medicine Humans Obesity Insulin-Like Growth Factor I Child business.industry Insulin Case-control study Receptors Somatomedin medicine.disease Body Height Insulin-Like Growth Factor Binding Protein 1 030104 developmental biology Insulin-Like Growth Factor Binding Protein 3 Case-Control Studies Child Preschool Female business Body mass index |
| Zdroj: | Growth hormoneIGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society. 39 |
| ISSN: | 1532-2238 |
| Popis: | Objectives Obese children are often taller than age-matched subjects. Reports on GH and IGF-I levels in obese individuals are controversial, with normal and reduced GH-IGF-I levels having been reported in this group of patients. Thus, the aim of this study was to analyse insulin-like growth factor type 1 receptor (IGF-IR) mRNA expression in obese children. Methods Forty-seven pre-pubertal children were included in this study: 29 were obese and taller than their target height, and 18 were normal eutrophic controls. Fasting blood samples were collected for IGF-IR mRNA expression in isolated lymphocytes and serum IGF-I, ALS, IGFBP-3, and IGFBP-1 concentration analysis. Results Relative IGF-IR gene expression (2− ΔΔCT) was significantly (P = 0.025) higher in obese children (median 1.87) than in controls (1.15). Fourteen of the 29 obese subjects showed 2− ΔΔCT values greater than or equal to 2, while only 2 individuals in the control group showed values above 2 (P = 0.01). Obese children showed significantly (P = 0.01) higher IGF-I concentrations than the control group (237 ng/ml and 144 ng/ml, respectively). Among obese patients, 65.5% had IGF-I values above the 75 percentile of the control group (P = 0.02). ALS concentration was significantly (P = 0.04) higher in the obese group, while IGFBP-3 levels were similar in obese and control children. IGFBP-1 concentration was lower in obese children, while insulin levels and HOMA-IR index were higher than in controls. Conclusions The higher IGF-IR mRNA expression observed in obese children, associated with the higher IGF-I and ALS and the lower IGFBP-1 levels, suggest that the higher stature observed in these children may be due to increased IGF-I bioactivity. |
| Databáze: | OpenAIRE |
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