The effect of histone H1 and DNA methylation on transcription
| Autor: | John P. Goddard, Roger L. P. Adams, Colin A. Johnson |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
RNA
Transfer Leu Transcription Genetic RNA Transfer Arg Biology Cell Fractionation Methylation Biochemistry Histones Epigenetics of physical exercise Histone H1 Histone H2A Histone methylation Escherichia coli Humans Histone code Histone octamer Molecular Biology Cell Nucleus Pioneer factor DNA Templates Genetic Cell Biology Molecular biology Ammonium Sulfate Histone methyltransferase Plasmids Research Article |
| Zdroj: | Biochemical Journal. 305:791-798 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3050791 |
| Popis: | We have previously shown that DNA methylation acts as a focus for the formation of inactive chromatin in vivo. We have investigated the mechanism further by in vitro transcription of a template containing two tRNA genes and an extensive (G+C)-rich sequence characteristic of a CpG island. The extent of transcription from the unmethylated or fully methylated template was assayed in the presence of varied levels of histone H1. The transcriptional activity of both templates was inhibited by increasing amounts of histone H1, although inhibition with the methylated template occurs at a lower H1:DNA ratio. The H1c variant shows the greatest preferential inhibition of the methylated template. We demonstrated that histone H1 complexed to DNA is one of the factors that inhibits transcription by preventing the formation of initiation complexes, particularly on methylated template, rather than the formation of disordered H1.DNA aggregates. |
| Databáze: | OpenAIRE |
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