Application of FTIR microspectroscopy for characterization of biomolecular changes in human melanoma cells treated by sesamol and kojic acid
| Autor: | Natthida Weerapreeyakul, Montra Srisayam, Sahapat Barusrux, Waraporn Tanthanuch, Kanjana Thumanu |
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| Rok vydání: | 2013 |
| Předmět: |
Cell Survival
Tyrosinase Dermatology Biochemistry Melanin chemistry.chemical_compound Phenols Cell Line Tumor Spectroscopy Fourier Transform Infrared medicine Humans Benzodioxoles Sesamol Molecular Biology Melanoma Melanosome chemistry.chemical_classification Principal Component Analysis Hyperpigmentation Enzyme chemistry Pyrones Nucleic acid medicine.symptom Kojic acid |
| Zdroj: | Journal of dermatological science. 73(3) |
| ISSN: | 1873-569X |
| Popis: | Background Hyperpigmentation is aesthetic undesirable. Sesamol and the standard antimelanogenic agent (kojic acid) were shown to hinder melanogenesis by blocking tyrosinase and reducing melanin content. Objective The FTIR microspectroscopy was used in an attempt to find a novel method to define biological alternation in a melanogenesis inhibition of sesamol and kojic acid. Methods Tyrosinase inhibition and melanin content of sesamol and kojic acid were evaluated. The FTIR microspectroscopy was adopted to define the vibrational characteristic involved with the melanogenesis in the untreated SK-MEL2 cells vs. the sesamol- and kojic-treated SK-MEL2 cells. Results Sesamol and kojic acid inhibited mushroom tyrosinase at IC 50 of 0.33 μg/ml and 6.1 ± 0.4 μg/ml, respectively. Moreover, 30 μg/ml sesamol inhibited 23.55 ± 8.25% cellular tyrosinase activity in human SK-MEL2 cells, while 600 μg/ml kojic acid inhibited 33.9 ± 1.4% cellular tyrosinase activity in the same cells. In the SK-MEL2-treated with two inhibitors, the FTIR spectra assigned to the lipid and nucleic acid bands were significantly depleted with the secondary protein structure shifted to a more β-pleated secondary protein one. Conclusion Both sesamol and kojic acid display a similar pattern of antimelanogenesis activity albeit to a different degree. The mechanism of their whitening effect may be via the alteration of (a) the enzyme conformation disallowing the ordinary enzyme–substrate interaction and maybe (b) the integrity of the lipid-containing melanosome. Our results support the alternative use of FTIR microspectroscopy as a simple and reagent-free method for characterization of biomolecular changes in human melanoma cells. |
| Databáze: | OpenAIRE |
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