The Molecular Mechanism and Neuroprotective Effect of Dihydrocapsaicin-Induced Mild Hypothermia After Cardiopulmonary Resuscitation in Rats
| Autor: | Shiwu Zhang, Xiaopeng Zhong, Xiujuan Wang, Po Ding, Fei Fei, Mancui Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
endocrine system Vasopressin medicine.medical_specialty Central nervous system Drug Evaluation Preclinical Hypothalamus TRPV1 TRPV Cation Channels Apoptosis 030204 cardiovascular system & hematology Critical Care and Intensive Care Medicine Rats Sprague-Dawley Random Allocation 03 medical and health sciences 0302 clinical medicine Hypothermia Induced Internal medicine medicine Animals Cerebral Cortex Asphyxia TUNEL assay Caspase 3 business.industry Hypothermia Cardiopulmonary Resuscitation Neuroprotection Heart Arrest Anesthesiology and Pain Medicine medicine.anatomical_structure Endocrinology Cerebral cortex Anesthesia Calcium Capsaicin medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Therapeutic Hypothermia and Temperature Management. 8:76-82 |
| ISSN: | 2153-7933 2153-7658 |
| DOI: | 10.1089/ther.2017.0032 |
| Popis: | To investigate the molecular mechanism of dihydrocapsaicin (DHC)-induced mild hypothermia in rats, and to compare its protective effect on the central nervous system with that of a conventional method of inducing hypothermia, 24 healthy male Sprague Dawley rats were randomly divided into four groups based on the following conditions: control group, cardiopulmonary resuscitation (CPR) group, body surface cooling group, and DHC group. Tracheal clipping was used to mimic asphyxia arrest. Rats were assessed for their neurological deficit scores. After sacrifice, immunohistochemical staining was used to examine caspase-3 expression in the cerebral cortex and TRPV1 (transient receptor potential vanilloid subfamily, member 1) expression in the hypothalamus. Terminal TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining was used to evaluate cell apoptosis in the cerebral cortex. Furthermore, intracellular Ca2+ concentration in the hypothalamus and arginine vasopressin (AVP) concentration in ventral septal t... |
| Databáze: | OpenAIRE |
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