Identification and Characterization of Orientia chuto in Trombiculid Chigger Mites Collected from Wild Rodents in Kenya
| Autor: | John N. Waitumbi, Tom Gilbreath, Surachai Leepitakrat, Beth Mutai, Clement Masakhwe, Elizabeth Wanja, David Abuom, Santos Yalwala, Nutthanun Auysawasi, Gathii Kimita, Piyada Linsuwanon |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Orientia tsutsugamushi Epidemiology 030106 microbiology 030231 tropical medicine Zoology Animals Wild Rodentia Scrub typhus Trombiculidae Polymerase Chain Reaction Neotrombicula Rodent Diseases 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Crocidura RNA Ribosomal 16S medicine Animals Phylogeny Rickettsieae biology Phylogenetic tree Nucleic Acid Hybridization Sequence Analysis DNA bacterial infections and mycoses Acomys wilsoni biology.organism_classification medicine.disease Orientia Kenya Scrub Typhus Mastomys |
| Popis: | We present data that concurs with the reported geographical expansion of scrub typhus outside the "Tsutsugamushi Triangle" and addition of Orientia chuto as a second species in the Orientia genus. Wild rodents were caught in Marigat, Baringo County, Kenya, and ectoparasites, including chiggers, were recovered. Rodent and chigger species were identified by taxonomic features. DNA was extracted from the chiggers and used to amplify and/or sequence the 47-kDa high temperature transmembrane protein (TSA47), the 56-kDa type-specific antigen (TSA56), and the 16S rRNA (rrs) Orientia genes. The main rodent hosts identified were Acomys wilsoni, Crocidura sp., and Mastomys natalensis, which accounted for 59.2% of the total collection. Of these, A. wilsoni and M. natalensis harbored most of the chiggers that belonged to the Neotrombicula and Microtrombicula genera. A pool of chiggers from one of M. natalensis was positive for Orientia by TSA47 PCR, but Orientia did not amplify with the TSA56 primers. On sequencing the 850 bp of the TSA47 gene, the closest phylogenetic relative was O. chuto, with 97.65% sequence homology compared to 84.63 to 84.76% for O. tsutsugamushi 16S rRNA deep sequencing also revealed O. chuto as the closest phylogenetic relative, with 99.75% sequence homology. These results and the existing immunological and molecular reports are strongly suggestive of the existence of Orientia species in Kenya. |
| Databáze: | OpenAIRE |
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