Transforming Growth Factor-induced Protein Promotes NF-κB-mediated Angiogenesis during Postnatal Lung Development
| Autor: | Simon J. Conway, Racquel Domingo-Gonzalez, Paige Snider, Gray Umbach, Ruby E. Dewi, Cristiana Iosef, Mar Janna Dahl, Donald M. Null, Kurt H. Albertine, Sarah C. Heilshorn, Min Liu, Sha Fu, Shailaja Rao, Cristina M. Alvira |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Angiogenesis Clinical Biochemistry Neovascularization Physiologic Nitric Oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Colony-Stimulating Factors Cell Movement Transforming Growth Factor beta medicine Animals Molecular Biology Lung Extracellular Matrix Proteins Sheep business.industry NF-kappa B Editorials Endothelial Cells NF-κB Cell Biology respiratory system medicine.disease Integrin alphaVbeta3 Mice Inbred C57BL Pulmonary Alveoli 030104 developmental biology medicine.anatomical_structure 030228 respiratory system Bronchopulmonary dysplasia chemistry Animals Newborn Cancer research Premature Birth business Transforming growth factor |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology |
| ISSN: | 1535-4989 |
| Popis: | Pulmonary angiogenesis is a key driver of alveolarization. Our prior studies showed that NF-κB promotes pulmonary angiogenesis during early alveolarization. However, the mechanisms regulating temporal-specific NF-κB activation in the pulmonary vasculature are unknown. To identify mechanisms that activate proangiogenic NF-κB signaling in the developing pulmonary vasculature, proteomic analysis of the lung secretome was performed using two-dimensional difference gel electrophoresis. NF-κB activation and angiogenic function was assessed in primary pulmonary endothelial cells (PECs) and TGFBI (transforming growth factor-β-induced protein)-regulated genes identified using RNA sequencing. Alveolarization and pulmonary angiogenesis was assessed in wild-type and |
| Databáze: | OpenAIRE |
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