Development of a Murine Model of Pyogenic Flexor Tenosynovitis
| Autor: | Bowen Qiu, Constantinos Ketonis, Alayna E. Loiselle, Justin Cobb |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Pathology medicine.medical_specialty medicine.medical_treatment H&E stain medicine.disease_cause Mice 03 medical and health sciences 0302 clinical medicine In vivo medicine Animals Bioluminescence imaging Clinical significance Orthopedics and Sports Medicine Therapeutic Irrigation Saline 030222 orthopedics Debridement business.industry Weight change Tenosynovitis General Medicine Staphylococcal Infections Pathophysiology Staining Anti-Bacterial Agents Mice Inbred C57BL Disease Models Animal Tendon sheath 030104 developmental biology Histopathology Surgery business |
| DOI: | 10.1101/2020.02.07.925339 |
| Popis: | Background Pyogenic flexor tenosynovitis is a debilitating infection of the hand flexor tendon sheath with high morbidity despite standard treatments of empiric antibiotics with irrigation and debridement. In vivo studies in the available literature have used avian models, but these models are difficult to scale and maintain. The purpose of this study was to demonstrate the plausibility of a murine model of pyogenic flexor tenosynovitis utilizing bioluminescence imaging and tissue analysis at harvest. Methods A 2-μL inoculate of bioluminescent Xen29 Staphylococcus aureus or sterile phosphate-buffered saline solution (sPBS) was administered to the tendon sheath of 36 male C57BL/6J mice. The infectious course was monitored by bioluminescence imaging (BLI) via an in vivo imaging system, gross anatomic deformity, and weight change. The infected hind paws were harvested at 4 time points: 24 hours, 72 hours, 1 week, and 2 weeks for histological analysis using Alcian blue, hematoxylin, and Orange-G staining. Two-way analysis of variance with the Sidak multiple comparison test was used to assess differences in bioluminescence and weight at each time point. Results The infected cohort displayed significantly elevated bioluminescence values, had reductions in weight, and exhibited swelling of the infected digit throughout the course of infection. By day 4, most infected mice saw a substantial decrease in BLI signal intensity; however, 2 infected mice exhibited persistent BLI intensity through day 14. Histological analysis of the infected cohort showed tissue disorganization and the presence of a cellular infiltrate in and around the flexor tendon sheath. Conclusions A murine model of pyogenic flexor tenosynovitis is possible and can serve as an experimental platform for further investigation of the pathophysiology of pyogenic flexor tenosynovitis. Clinical relevance This animal model can be utilized in elucidating the basic molecular and/or cellular mechanisms of pyogenic flexor tenosynovitis while simultaneously evaluating novel therapeutic strategies. |
| Databáze: | OpenAIRE |
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