Amplified 16S ribosomal DNA restriction analysis for identification of Avibacterium paragallinarum
| Autor: | Alfredo Mendoza-Espinoza, Amparo I. Zavaleta, Ysabel Koga |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Restriction Mapping Biology medicine.disease_cause DNA Ribosomal law.invention Microbiology chemistry.chemical_compound Food Animals law RNA Ribosomal 16S medicine Animals Ribosomal DNA Escherichia coli Ornithobacterium rhinotracheale Polymerase chain reaction Genetics General Immunology and Microbiology Building and Construction DNA Restriction Enzymes Ribosomal RNA medicine.disease 16S ribosomal RNA chemistry Animal Science and Zoology Female Restriction fragment length polymorphism Pasteurellaceae Chickens Nucleic Acid Amplification Techniques DNA |
| Zdroj: | Avian diseases. 52(1) |
| ISSN: | 0005-2086 |
| Popis: | A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying hens from different regions of Peru. The 16S rDNA was amplified by PCR using a pair of bacterial universal primers and restriction analysis of 16S rDNA sequences was done to select endonucleases with the highest number of cutting points inside the 16S rDNA. The DNA patterns with DdeI and RsaI endonucleases were identical for the 19 A. paragallinarum strains, but differed from those obtained for Ornithobacterium rhinotracheale, a bacterium with a high genetic and phenotypic resemblance to A. paragallinarum, as well as from Escherichia coli, a bacterium associated with infectious coryza. The ARDRA method could prove to be valuable for molecular identification of A. paragallinarum, a microorganism implicated in respiratory diseases in commercial birds. |
| Databáze: | OpenAIRE |
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