Capillary Separation: Micellar Electrokinetic Chromatography

Autor: Shigeru Terabe
Rok vydání: 2009
Předmět:
Zdroj: Annual Review of Analytical Chemistry. 2:99-120
ISSN: 1936-1335
1936-1327
Popis: Micellar electrokinetic chromatography (MEKC), a separation mode of capillary electrophoresis (CE), has enabled the separation of electrically neutral analytes. MEKC can be performed by adding an ionic micelle to the running solution of CE without modifying the instrument. Its separation principle is based on the differential migration of the ionic micelles and the bulk running buffer under electrophoresis conditions and on the interaction between the analyte and the micelle. Hence, MEKC's separation principle is similar to that of chromatography. MEKC is a useful technique particularly for the separation of small molecules, both neutral and charged, and yields high-efficiency separation in a short time with minimum amounts of sample and reagents. To improve the concentration sensitivity of detection, several on-line sample preconcentration techniques such as sweeping have been developed.
Databáze: OpenAIRE