Microsporidial infections due toEncephalitozoon intestinalisin non-HIV-infected patients with chronic diarrhoea
| Autor: | A. Khalid, Zaigham Abbas, Javed Yakoob, Shagufta Naz, Rustam Khan, Wasim Jafri, M. Asim Beg |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Diarrhea Male medicine.medical_specialty Carcinoma Hepatocellular Adolescent Epidemiology Polymerase Chain Reaction Gastroenterology law.invention Feces Young Adult Trichrome law Internal medicine medicine Carcinoma Humans DNA Fungal Polymerase chain reaction Aged Aged 80 and over Microscopy Staining and Labeling biology business.industry Liver Neoplasms Fungal genetics Encephalitozoon Enterocytozoon Middle Aged biology.organism_classification medicine.disease Encephalitozoon intestinalis Infectious Diseases Hepatocellular carcinoma Microsporidia Encephalitozoonosis Female business |
| Zdroj: | Epidemiology and Infection. 140:1773-1779 |
| ISSN: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268811002639 |
| Popis: | SUMMARYWe determined the prevalence of microsporidiaEnterocytozoon(Ent.)bieneusiandEncephalitozoon(E.)intestinalisinfection in patients with chronic diarrhoea and hepatocellular carcinoma (HCC). A total of 330 stool samples were examined from 171 (52%) patients with chronic diarrhoea, 18 (5%) with HCC while 141 (43%) were controls. Stool microscopy, polymerase chain reaction (PCR) with species-specific primers forEnt. bieneusiandE. intestinalisand sequencing were carried out. Microsporidia were found by trichrome staining in 11/330 (3%) andE. intestinalisby PCR in 13/330 (4%) whileEnt. bieneusiwas not detected. PCR forE. intestinaliswas positive in 8/171 (5%) stool samples from patients with chronic diarrhoea, 2/141 (1·4%) samples from healthy controls and in 3/18 (17%) samples from patients with HCC. In the chronic diarrhoea group,E. intestinaliswas positive in 4/171 (2·3%) (P=0·69) stool samples compared to 2/18 (11%) (P=0·06) in the HCC group and 2/141 (1·4%) from healthy controls.E. intestinalisinfection was significantly associated with chronic diarrhoea and HCC in these patients who were negative for HIV. Stool examination with trichrome or species-specific PCR for microsporidia may help establish the cause of chronic diarrhoea. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|