Contribution of Flavonoids and Iridoids to the Hypoglycaemic, Antioxidant, and Nitric Oxide (NO) Inhibitory Activities of Arbutus unedo L
| Autor: | Rosa Tundis, Giuseppe Antonio Malfa, Brigitte Deguin, Maria Concetta Tenuta, Monica Rosa Loizzo, Rosaria Acquaviva, Marco Bonesi, Chouaha Bouzidi, Annabelle Dugay |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant functional products Dried fruit Physiology DPPH medicine.medical_treatment Clinical Biochemistry chemical profiles metabolic diseases Biochemistry Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Maceration (wine) Food science Molecular Biology Chemical profiles Extraction procedures Functional products Metabolic diseases Strawberry tree ABTS lcsh:RM1-950 food and beverages Cell Biology lcsh:Therapeutics. Pharmacology 030104 developmental biology strawberry tree extraction procedures chemistry 030220 oncology & carcinogenesis Kaempferol Ellagic acid |
| Zdroj: | Antioxidants Volume 9 Issue 2 Antioxidants, Vol 9, Iss 2, p 184 (2020) |
| ISSN: | 2076-3921 |
| Popis: | This study aims at investigating the contribution of two classes of compounds, flavonoids and iridoids, to the bioactivity of Arbutus unedo L. leaves and fruits. The impact of different extraction procedures on phytochemicals content and hypoglycemic, antioxidant, and nitric oxide (NO) inhibitory activities of A. unedo fresh and dried plant materials was investigated. Ellagic acid 4-O-&beta D-glucopyranoside, kaempferol 3-O-glucoside, and norbergenin were identified for the first time in this genus by using liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-QTOF-MS). Three iridoids (gardenoside, geniposide, unedoside) are specifically identified in the leaves. Interestingly, asperuloside was extracted only from dried fruits by ethanol with Soxhlet apparatus. Extracts were screened for their potential antioxidant activities by using the 2,2&prime azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Activity Power (FRAP), and &beta carotene bleaching tests. Based on the Global Antioxidant Score (GAS) calculation, the most promising antioxidant extract was obtained by hydroalcoholic maceration of dried leaves that showed half maximal inhibitory concentration (IC50) of 0.42 and 0.98 &mu g/mL in ABTS and DPPH assays, respectively. The hypoglycaemic activity was investigated by &alpha amylase and &alpha glucosidase inhibition tests. Extracts obtained by ethanol ultrasound extraction of fresh leaves and hydroalcoholic maceration of fresh fruits (IC50 of 19.56 and 28.42 &mu g/mL, respectively) are more active against &alpha glucosidase than the positive control acarbose (IC50 of 35.50 &mu g/mL). Fruit extracts exhibited the highest anti-inflammatory activity. |
| Databáze: | OpenAIRE |
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