THE INFLUENCE OF VARIOUS FIXATIVES ON THE IMMUNOHISTOCHEMICAL DEMONSTRATION OF A NUMBER OF PLASMA PROTEINS AND ONCOFETAL PROTEINS IN PARAFFIN EMBEDDED MATERIAL
| Autor: | Marianne Jacobsen, Grete Krag Jacobsen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Antigenicity
Placenta Biology Chorionic Gonadotropin Fixatives chemistry.chemical_compound Human placental lactogen Immunology and Microbiology (miscellaneous) Antigens Neoplasm Pregnancy Humans Prealbumin Paraformaldehyde Serum Albumin Bouin solution Fixative Fixation (histology) General Immunology and Microbiology Pregnancy-Specific beta 1-Glycoproteins Histological Techniques Transferrin Albumin Blood Proteins General Medicine Placental Lactogen Blood proteins Liver Biochemistry chemistry Female alpha-Fetoproteins |
| Zdroj: | Acta Pathologica Microbiologica Scandinavica Series A :Pathology. :461-468 |
| ISSN: | 0108-0164 |
| Popis: | The influence of different fixatives on the antigenicity of alpha-fetoprotein (AFP), albumin, prealbumin, transferrin, ferritin, human chorionic gonadotropin (HCG), specific pregnancy beta 1-glycoprotein (SP1) and human placental lactogen (HPL) was studied. No single fixative was superior for all the antigens investigated. For intracellular antigens the best all-around fixatives were, however, 10% buffered formalin, 4% buffered paraformaldehyde, 10% formol-calcium and ethanol acetic acid (EA), cold processed. The preferable fixatives were for AFP: Bouińs fixative, B-5 and EA; for albumin: 10% formol-calcium; for prealbumin: Zambonis fixative and B-5; for transferrin: EA; for ferritin: 10% buffered formalin, 4% buffered paraformaldehyde, Lilliés fixative, Zambonis fixative and EA; for HCG: Bouińs fixative and B-5; for SP1: Lilliés fixative and EA; and for HPL: 4% buffered paraformaldehyde and Lilliés fixative. The preservation of extracellular antigens was different from the above mentioned intracellular ones. Thus fixation in 10% buffered formalin gave poor results while the coagulant fixatives such as Bouińs fixative, B-5 and Clelands fixative in general showed good preservation of the antigenicity of the extracellular antigens. |
| Databáze: | OpenAIRE |
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