Local FK506 delivery at the direct nerve repair site improves nerve regeneration
| Autor: | Jay Agarwal, Himanshu J. Sant, Pratima Labroo, Sierra Erickson, Brett Davis, Jill E. Shea, David Hilgart, Bruce K. Gale, Joshua N. Burton |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Physiology Polyesters 030105 genetics & heredity Neurosurgical Procedures Tacrolimus Mice 03 medical and health sciences Cellular and Molecular Neuroscience Gastrocnemius muscle 0302 clinical medicine Dorsal root ganglion Peripheral Nerve Injuries Ganglia Spinal Physiology (medical) Direct repair Neurites polycyclic compounds Animals Medicine Muscle Skeletal Nerve repair Electromyography business.industry organic chemicals Regeneration (biology) Organ Size Repair site Sciatic Nerve Controlled release Axons Muscle Denervation Nerve Regeneration enzymes and coenzymes (carbohydrates) medicine.anatomical_structure Delayed-Action Preparations Anesthesia cardiovascular system Neurology (clinical) Sciatic nerve business Immunosuppressive Agents 030217 neurology & neurosurgery |
| Zdroj: | Muscle & Nerve. 60:613-620 |
| ISSN: | 1097-4598 0148-639X |
| DOI: | 10.1002/mus.26656 |
| Popis: | Introduction The objective of this study is to assess the efficacy of local tacrolimus (FK506) delivery to improve outcomes in the setting of nerve transection injury. Methods FK506 embedded poly(lactide-co-caprolactone) films capable of extended, localized release of FK506 were developed. FK506 rate of release testing and bioactivity assay was performed. Mouse sciatic nerve transection and direct repair model was used to evaluate the effect extended, local delivery of FK506 had on nerve regeneration outcomes. Results Linear release of FK506 was observed for 30 days and released FK506 matched control levels of neurite extension in the dorsal root ganglion assay. Groups treated with local FK506 had greater gastrocnemius muscle weight, foot electromyogram, and number of axons distal of the repair site than non-FK506 groups. Discussion Results of this study indicate that extended, localized delivery of FK506 to nerve injuries can improve nerve regeneration outcomes in a mouse sciatic nerve transection and repair. |
| Databáze: | OpenAIRE |
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