Systemic and local induction of anArabidopsisthionin gene by wounding and pathogens
| Autor: | Claus Wasternack, Alberto Vignutelli, Klaus Apel, Holger Bohlmann |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Plant Molecular Sequence Data Arabidopsis Plant Science chemistry.chemical_compound Gene Expression Regulation Plant Gene expression Genetics Arabidopsis thaliana Cloning Molecular Promoter Regions Genetic Gene Glucuronidase Plant Proteins Sequence Deletion Pathogenesis-related protein Methyl jasmonate Base Sequence biology Arabidopsis Proteins Gene Expression Regulation Developmental Cell Biology Plants Genetically Modified biology.organism_classification Thionin Cell biology chemistry Agrobacterium tumefaciens Octadecanoid pathway Antimicrobial Cationic Peptides |
| Zdroj: | The Plant Journal. 14:285-295 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1046/j.1365-313x.1998.00117.x |
| Popis: | The Arabidopsis Thi2.1 thionin gene was cloned and sequenced. The promoter was fused to the uidA gene and stably transformed into Arabidopsis to study its regulation. GUS expression levels correlated with the steady-state levels of Thi2.1 mRNA, thus demonstrating that the promoter is sufficient for the regulation of the Thi2.1 gene. The sensitivity of the Thi2.1 gene to methyl jasmonate was found to be developmentally determined. Systemic and local expression could be induced by wounding and inoculation with Fusarium oxysporum f sp. matthiolae. A deletion analysis of the promoter identified a fragment of 325 bp upstream of the start codon, which appears to contain all the elements necessary for the regulation of the Thi2.1 gene. These results support the view that thionins are defence proteins, and indicate the possibility that resistance of Arabidopsis plants to necrotrophic fungal pathogens is mediated through the octadecanoid pathway. |
| Databáze: | OpenAIRE |
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