Inhibitory effect of STAT3 gene combined with CDDP on growth of human Wilms tumour SK-NEP-1 cells
| Autor: | Junhui Yuan, Junrong Wang, Haijiang Qu, Guangxian You, Caie Chen, Wenyi Li, Nina Zhang, Feng Pan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
STAT3 Transcription Factor
0301 basic medicine Cell Biophysics S48 S42 Wilms Tumor Biochemistry STAT3 Mice 03 medical and health sciences 0302 clinical medicine Bcl-2-associated X protein Cell Line Tumor Heat shock protein medicine Animals Humans Endoplasmic Reticulum Chaperone BiP Molecular Biology Heat-Shock Proteins bcl-2-Associated X Protein Cisplatin Original Paper cisplatin (CDDP) glucose regulatory protein 78 (GRP78) biology Wilms tumour (WT) Wilms' tumor Cell Biology medicine.disease Original Papers Xenograft Model Antitumor Assays Molecular biology Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure S39 BAX 030220 oncology & carcinogenesis biology.protein Cancer research STAT protein Immunohistochemistry medicine.drug |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| DOI: | 10.1042/bsr20160072 |
| Popis: | To investigate the effects of signal transducer and activator of transcription 3 (STAT3) combined with cisplatin (CDDP) on the growth of human Wilms tumour (WT) SK-NEP-1 cell subcutaneous xenografts in nude mice and the possible mechanisms. Human WT SK-NEP-1 cells were subcutaneously transplanted to establish the BALB/c nude mice xenograft model. Mice were randomly divided into five groups: blank control group, adenovirus control group (NC group), STAT3 group, CDDP group and STAT3 plus CDDP group (combination group). Tumour volume and tumour weight were observed during the therapeutic process. The expression levels of STAT3, glucose regulatory protein 78 (GRP78) and BCL2-associated X protein (BAX) were evaluated by immunohistochemical analysis. Compared with the STAT3 group or CDDP group, the tumour weight and volume was significantly reduced in the combination group (P 0.05). Immunohistochemical analysis showed that STAT3, GRP78 and BAX protein levels in the combination group were significantly higher than those in STAT3 group and CDDP group (P |
| Databáze: | OpenAIRE |
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