Identification of cis- and trans-regulatory variation modulating microRNA expression levels in human fibroblasts
| Autor: | Audrey Letourneau, Stylianos E. Antonarakis, Samuel Deutsch, Emilie Falconnet, Corinne Gehrig, Christelle Borel, Charles E. Vejnar, Eugenia Migliavacca, Stephen B. Montgomery, Antigone S. Dimas, Maryline Gagnebin, Emmanouil T. Dermitzakis, Yann Dupré, Homa Attar |
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| Rok vydání: | 2011 |
| Předmět: |
Quantitative Trait Loci
Quantitative trait locus Biology Cell Line 03 medical and health sciences MicroRNAs/genetics/metabolism 0302 clinical medicine microRNA Genetics Gene silencing Humans ddc:576.5 RNA Processing Post-Transcriptional Gene Genetics (clinical) 030304 developmental biology Regulation of gene expression Fibroblasts/metabolism 0303 health sciences Research Gene Expression Profiling Infant Newborn Genetic Variation Fibroblasts Phenotype Gene expression profiling Europe MicroRNAs Enhancer Elements Genetic Gene Expression Regulation Expression quantitative trait loci Quantitative Trait Loci/genetics 030217 neurology & neurosurgery Genome-Wide Association Study |
| Zdroj: | Genome research Genome Research, Vol. 21, No 1 (2011) pp. 68-73 |
| ISSN: | 1088-9051 |
| DOI: | 10.1101/gr.109371.110 |
| Popis: | MicroRNAs (miRNAs) are regulatory noncoding RNAs that affect the production of a significant fraction of human mRNAs via post-transcriptional regulation. Interindividual variation of the miRNA expression levels is likely to influence the expression of miRNA target genes and may therefore contribute to phenotypic differences in humans, including susceptibility to common disorders. The extent to which miRNA levels are genetically controlled is largely unknown. In this report, we assayed the expression levels of miRNAs in primary fibroblasts from 180 European newborns of the GenCord project and performed association analysis to identify eQTLs (expression quantitative traits loci). We detected robust expression for 121 miRNAs out of 365 interrogated. We have identified significant cis- (10%) and trans- (11%) eQTLs. Furthermore, we detected one genomic locus (rs1522653) that influences the expression levels of five miRNAs, thus unraveling a novel mechanism for coregulation of miRNA expression. |
| Databáze: | OpenAIRE |
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