Effect and Mechanism of Sophoridine to suppress Hepatocellular carcinoma in vitro and vivo
| Autor: | HaiYang Wang, Ning Liu, Jian Xu, Shunwu Chang, BaoChun Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Carcinoma Hepatocellular Cell Mice Nude Pharmacology Flow cytometry 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Alkaloids Liver Neoplasms Experimental Matrine Cell Line Tumor medicine Animals Humans Matrines Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation medicine.diagnostic_test Dose-Response Relationship Drug Chemistry Cell growth General Medicine Hep G2 Cells Xenograft Model Antitumor Assays Tumor Burden 030104 developmental biology medicine.anatomical_structure Treatment Outcome Apoptosis 030220 oncology & carcinogenesis Wound healing Quinolizines |
| Zdroj: | Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 95 |
| ISSN: | 1950-6007 |
| Popis: | The aim of this study is to explain effect and mechanism of Sophoridine to suppress Hepatocellular carcinoma in vitro and vivo.In vitro experiment, the HepG2 cells were divided into 5 groups: 0μg/mL Sophoridine treated group (0 μg/mL group); 10μg/mL matrine treated group (10μg/mL group); 20μg/mL matrine treated group (20μg/mL group) and 10μg/mL Paclitaxel treated group (Positive drug group). Measuring the cell proliferation of difference groups by MTS assay; evaluating cell apoptosis of difference by flow cytometry; the cell invasion and migration abilities of difference HepG2 cells were measured by transwell and wound healing testing; measuring the relative proteins expression in difference groups. In vovo experiment, the nude mice were divided into 5 groups: 0μg/mL, 5μg/mL, 10μg/mL, 20μg/mL and Positive drug groups, after executing, taking the tumor tissue from nude mice of difference groups, measuring the tumor volume and weight; evaluating the PTEN protein expression in tumor tissue by Immunohistochemistry (IHC).In the cell experiments, Compared with 0μg/mL group, cell proliferation rates were significantly reduced, cell aopotosis were significantly increased and invasion and wound healing abilities were significantly decreased in marine treated groups with dose-dependent (P0.05, respectively). In the nude mice experiment, the tumor volume and weight of matrine treated groups were significantly decreased compared with 0 μg/mL group with dose-dependent (P0.05, respectively). And the PTEN protein expression of Sophoridine treated groups were significantly decreased compared with 0μg/mL group with dose-dependent (P0.05, respectively).Sophoridine had anti-cance effects to suppress HepG2 activities by regulation PTEN/PI3K/AKT, Caspase-3/-9 and MMP-2/-9 signaling pathway. |
| Databáze: | OpenAIRE |
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