Decreased integrin gene expression in patients with MS responding to interferon-β treatment
| Autor: | A. Pantalone, L. Liberati, Alessandra Lugaresi, Carla Iarlori, Laura Bonanni, Christina M. Caporale, D. Farina, Domenico Gambi, G. De Luca, Paolo A. Muraro |
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| Přispěvatelé: | Muraro, P. A., Liberati, L., Bonanni, L., Pantalone, A., Caporale, C. M., Iarlori, C., De Luca, G., Farina, D., Lugaresi, A., Gambi, D. |
| Rok vydání: | 2004 |
| Předmět: |
Male
Integrins T-Lymphocytes Integrin alpha4beta1 Pharmacology Gene expression Leukocyte functional antigen-1 Immunology and Allergy Lymphocyte function-associated antigen 1 Interferon-β Blood-brain barrier biology Cell adhesion molecule hemic and immune systems Middle Aged Lymphocyte Function-Associated Antigen-1 Real-time polymerase chain reaction Neurology Fluorescence-activated cell sorting Female Interferon beta-1a LFA-1 medicine.drug Adult Adhesion molecule Adolescent VLA-4 Leukocyte adhesion molecule FACS Immunology Integrin Dose-Response Relationship Immunologic Down-Regulation FITC IFN-β Multiple Sclerosis Relapsing-Remitting Adjuvants Immunologic medicine Humans Multiple sclerosi RNA Messenger Cell Membrane MS Interferon-beta Fluorescein-iso-thiocyanate biology.protein Neurology (clinical) BBB |
| Zdroj: | Journal of Neuroimmunology. 150:123-131 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/j.jneuroim.2004.01.002 |
| Popis: | Interferon-β (IFN-β) ameliorates disease course in a subset of patients with MS. The reasons for heterogeneity of clinical responses, however, are unclear. We assessed possible effects of IFN-β on the gene expression of the leukocyte adhesion molecules VLA-4 and LFA-1 during the first year of treatment of 50 patients with relapsing-remitting MS who showed differential clinical responses. We observed a significant reduction of VLA-4 (P=0.002) and LFA-1 (P=0.03) mRNA expression compared to baseline in first-year clinical responders (n=22). In contrast, first-year IFN-β non-responders (n=28) had unchanged levels of VLA-4 and LFA-1. In vitro treatment of PBMC with IFN-β indicated a direct effect on transcription of the integrins' genes. Transcriptional downmodulation of adhesion molecules during IFN-β treatment may contribute to its mode of action in MS. © 2004 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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