Production of the Subdomains of the Plasmodium falciparum Apical Membrane Antigen 1 Ectodomain and Analysis of the Immune Response
| Autor: | Bader B. Fileta, P. V. Lalitha, J. D. Haynes, David E. Lanar, Sheetij Dutta, Arnoldo Barbosa, Lisa A. Ware, Charles E. White, J. K. Moch |
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| Rok vydání: | 2004 |
| Předmět: |
Erythrocytes
Plasmodium falciparum Immunology Protozoan Proteins Antibodies Protozoan Antigens Protozoan Biology Microbiology Gentamicin protection assay Antigen Malaria Vaccines Escherichia coli Animals Malaria Falciparum Apical membrane antigen 1 Membrane Proteins biology.organism_classification Virology Molecular biology Recombinant Proteins Transmembrane protein Infectious Diseases Ectodomain Membrane protein biology.protein Immunization Parasitology Rabbits Fungal and Parasitic Infections Antibody |
| Zdroj: | Infection and Immunity. 72:4464-4470 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.72.8.4464-4470.2004 |
| Popis: | The apical membrane antigen 1 of Plasmodium falciparum is one of the leading candidate antigens being developed as a vaccine to prevent malaria. This merozoite transmembrane protein has an ectodomain that can be divided into three subdomains (D I, D II, and D III). We have previously expressed a major portion of this ectodomain and have shown that it can induce antibodies that prevent merozoite invasion into red blood cells in an in vitro growth and invasion assay. To analyze the antibody responses directed against the individual subdomains, we constructed six different genes that express each of the domains separately (D I, D II, or D III) or in combination with another domain (D I+II, D II+III, or D I+III). These proteins were purified and used to immunize rabbits to raise construct-specific antibodies. We demonstrated that D I+II induced a significant amount of the growth-inhibitory antibodies active in the growth and invasion assay. |
| Databáze: | OpenAIRE |
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