Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System

Autor: Jumana R. Alhamdi, Nadia Benkirane-Jessel, Marja M. Hurley, Emily Jacobs, Gloria Gronowicz, Liisa T. Kuhn
Přispěvatelé: University of Connecticut Health Center [Farmington], Nanomédecine Régénérative (NanoRegMed), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), univOAK, Archive ouverte
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
[CHIM.POLY] Chemical Sciences/Polymers
polyelectrolyte multilayers
02 engineering and technology
engineering.material
lcsh:Technology
Apatite
Article
Barrier layer
03 medical and health sciences
Coating
biomimetic calcium phosphate
General Materials Science
lcsh:Microscopy
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
lcsh:QC120-168.85
chemistry.chemical_classification
lcsh:QH201-278.5
lcsh:T
Biomolecule
Substrate (chemistry)
cell-biomaterial interactions
021001 nanoscience & nanotechnology
Polyelectrolyte
[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials
030104 developmental biology
[CHIM.POLY]Chemical Sciences/Polymers
chemistry
lcsh:TA1-2040
visual_art
[SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Drug delivery
drug delivery
Biophysics
visual_art.visual_art_medium
engineering
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
lcsh:Descriptive and experimental mechanics
lcsh:Electrical engineering. Electronics. Nuclear engineering
lcsh:Engineering (General). Civil engineering (General)
0210 nano-technology
lcsh:TK1-9971
Layer (electronics)
bone like apatite
Zdroj: Materials
Volume 11
Issue 9
Materials, 2018, 11 (9), pp.1703. ⟨10.3390/ma11091703⟩
Materials, Vol 11, Iss 9, p 1703 (2018)
ISSN: 1996-1944
DOI: 10.3390/ma11091703
Popis: International audience; Recently, the benefit of step-wise sequential delivery of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 from a bioinspired apatite drug delivery system on mouse calvarial bone repair was demonstrated. The thicknesses of the nanostructured poly-L-Lysine/poly-L-Glutamic acid polyelectrolyte multilayer (PEM) and the bone-like apatite barrier layer that make up the delivery system, were varied. The effects of the structural variations of the coating on the kinetics of cell access to a cytotoxic factor delivered by the layered structure were evaluated. FGF-2 was adsorbed into the outer PEM, and cytotoxic antimycin-A (AntiA) was adsorbed to the substrate below the barrier layer to detect the timing of the cell access. While MC3T3-E1 osteoprogenitor cells accessed AntiA after three days, the RAW 264.7 macrophage access occurred within 4 h, unless the PEM layer was removed, in which case the results were reversed. Pits were created in the coating by the RAW 264.7 macrophages and initiated delivery, while the osteoprogenitor cell access to drugs occurred through a solution-mediated coating dissolution, at junctions between the islands of crystals. Macrophage-mediated degradation is therefore a mechanism that controls drug release from coatings containing bioinspired apatite.
Databáze: OpenAIRE
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