An improved amperometric determination of xanthine with xanthine oxidase nanoparticles for testing of fish meat freshness
| Autor: | Jyoti Ahlawat, Archana Joon, Ranjana Jaiwal, Vishakha Aggarwal, Chandra Shekhar Pundir |
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| Rok vydání: | 2021 |
| Předmět: |
Auxiliary electrode
Working electrode 02 engineering and technology Xanthine 01 natural sciences Reference electrode chemistry.chemical_compound Fish meat Electrical and Electronic Engineering Xanthine oxidase 010401 analytical chemistry technology industry and agriculture Engineering (General). Civil engineering (General) 021001 nanoscience & nanotechnology Amperometry 0104 chemical sciences Electronic Optical and Magnetic Materials chemistry Signal Processing Electrode Au electrode Xanthine oxidase nanoparticles TA1-2040 Xanthine biosensor 0210 nano-technology Biosensor Biotechnology Nuclear chemistry |
| Zdroj: | Sensing and Bio-Sensing Research, Vol 33, Iss, Pp 100437-(2021) |
| ISSN: | 2214-1804 |
| Popis: | The nanoparticles of xanthine oxidase (XODNPs) were prepared by desolvation method and characterized by transmission electron microscopy(TEM) and Fourier transform Infra-red spectroscopy(FTIR). These XODNPs were immobilized directly onto surface of Au electrode via covalent bond. An improved amperometric xanthine biosensor was fabricated using XODNPs modified Au electrode, as working electrode (WE), Ag/AgCl as reference electrode and Pt wire as auxiliary electrode connected through potentiostat.The biosensor showed optimum current at 0.25 V within 5 s, at pH 7.0 and 35 °C.The working range of biosensor was 0.01–1.0 μM with a limit of detection of 0.01 μM.The % recovery of added xanthine (1 and 5 μM) was 94.1 ± 0.2 and 94.8 ± 0.31 respectively. Within and between batch coefficient of variation (CV) were |
| Databáze: | OpenAIRE |
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