Administration of Lactococcus lactis strain Plasma induces maturation of plasmacytoid dendritic cells and protection from rotavirus infection in suckling mice
| Autor: | Tetsu Sugimura, Konomi Ohshio, Kenta Jounai, Yuji Morita, Daisuke Fujiwara |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Rotavirus Immunology Administration Oral Pancreatitis-Associated Proteins medicine.disease_cause Antiviral Agents Virus Rotavirus Infections Microbiology 03 medical and health sciences Mice In vivo medicine Immunology and Allergy Animals Cells Cultured Pharmacology Lamina propria Mice Inbred BALB C Mucous Membrane biology Lactococcus lactis Cell Differentiation Epithelial Cells Dendritic Cells biology.organism_classification Small intestine Animals Suckling Titer 030104 developmental biology medicine.anatomical_structure Animals Newborn Viperin Female Interferons |
| Zdroj: | International immunopharmacology. 56 |
| ISSN: | 1878-1705 |
| Popis: | Lactococcus lactis subsp. lactis JCM 5805 (LC-Plasma) is a strain of lactic acid bacteria (LAB) that activates murine and human plasmacytoid dendritic cells (pDCs) to express interferons (IFNs). Oral administration of LC-Plasma drastically decreased fatality levels caused by parainfluenza virus infection in a murine model. In this study, we investigated the anti-viral effects of oral administration of LC-Plasma using a suckling mouse model of rhesus rotavirus (RV) infection. LC-Plasma-fed mice showed improvement in retardation of body weight gain, fecal scores, and a reduction in RV titer in the feces when compared to control mice. The mechanism of anti-viral effects elicited by LC-Plasma administration was investigated using naive mice: in the LC-Plasma -fed mice, lamina propria (LP) pDCs resident in the small intestine were significantly matured and the proportion of pDCs was increased. The expression levels of anti-viral factors induced by IFNs, such as Isg15, Mx1, Oasl2 and Viperin, and an anti-bacterial factor Reg3γ, were up-regulated in the small intestinal epithelial cells (IECs) of LC-Plasma-fed mice. The specific LAB strain may affect the anti-viral immunological profile of IECs via maturation of LP pDCs, leading to protection from RV virus infection in vivo. |
| Databáze: | OpenAIRE |
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