Low-Volume Label-Free Detection of Molecule-Protein Interactions on Microarrays by Imaging Reflectometric Interferometry
| Autor: | Thomas Brandstetter, Christin Rath, Juergen Burger, Johannes Woehrle, Normann Kilb, Philipp A. Meyer, Guenter Roth, Nessim Ben Ammar, Gerald Urban, Guenther Proll, Florian Pröll |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Microfluidics Microscope slide Biotin 02 engineering and technology Sensitivity and Specificity 01 natural sciences Protein–protein interaction Optics Surface plasmon resonance Image resolution Plasmon business.industry 010401 analytical chemistry Thrombin Proteins Serum Albumin Bovine Quartz crystal microbalance Aptamers Nucleotide Microarray Analysis 021001 nanoscience & nanotechnology 0104 chemical sciences Computer Science Applications Medical Laboratory Technology Interferometry Streptavidin 0210 nano-technology business Protein Binding |
| Zdroj: | SLAS Technology. 22:437-446 |
| ISSN: | 2472-6303 |
| DOI: | 10.1177/2211068216657512 |
| Popis: | This system allows the high-throughput protein interaction analysis on microarrays. We apply the interference technology 1λ–imaging reflectometric interferometry (iRIf) as a label-free detection method and create microfluidic flow cells in microscope slide format for low reagent consumption and lab work compatibility. By now, most prominent for imaging label-free interaction analyses on microarrays are imaging surface plasmon resonance (SPR) methods, quartz crystal microbalance, or biolayer interferometry. SPR is sensitive against temperature drifts and suffers from plasmon crosstalk, and all systems lack array size (maximum 96 spots). Our detection system is robust against temperature drifts. Microarrays are analyzed with a spatial resolution of 7 µm and time resolution of ≤50 fps. System sensitivity is competitive, with random noise of |
| Databáze: | OpenAIRE |
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