Amplification of a Novel v- erb B-Related Gene in a Human Mammary Carcinoma
| Autor: | Matthias H. Kraus, CR King, Stuart A. Aaronson |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Breast Neoplasms
Receptors Cell Surface Molecular cloning Biology Proto-Oncogene Mas Cell Line Exon ErbB Epidermal growth factor Humans Amino Acid Sequence Cloning Molecular Peptide sequence Gene Multidisciplinary Base Sequence Gene Amplification Nucleic acid sequence DNA Neoplasm Oncogenes Protein-Tyrosine Kinases Molecular biology ErbB Receptors Gene Expression Regulation Female Protein Kinases Tyrosine kinase |
| Zdroj: | Science. 229:974-976 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.2992089 |
| Popis: | The cellular gene encoding the receptor for epidermal growth factor (EGF) has considerable homology to the oncogene of avian erythroblastosis virus. In a human mammary carcinoma, a DNA sequence was identified that is related to v-erbB but amplified in a manner that appeared to distinguish it from the gene for the EGF receptor. Molecular cloning of this DNA segment and nucleotide sequence analysis revealed the presence of two putative exons in a DNA segment whose predicted amino acid sequence was closely related to, but different from, the corresponding sequence of the erbB/EGF receptor. Moreover, this DNA segment identified a 5-kilobase transcript distinct from the transcripts of the EGF receptor gene. Thus, a new member of the tyrosine kinase proto-oncogene family has been identified on the basis of its amplification in a human mammary carcinoma. |
| Databáze: | OpenAIRE |
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