Ochratoxin A cytotoxicity on Madin-Darby canine kidney cells in the presence of alpha-tocopherol: Effects on cell viability and tight junctions
| Autor: | Federica Cheli, Cinzia Domeneghini, Eleonora Fusi, Antonella Baldi, V. Caprarulo, F. Vitari, Raffaella Rebucci, Carlotta Giromini, Luciano Pinotti |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Apoptosis Madin–Darby canine kidney cell mycotoxin occludin vitamin E Zo1 Cell Survival alpha-Tocopherol Biology Occludin Cell Line Tight Junctions 03 medical and health sciences chemistry.chemical_compound Dogs Food Animals Lactate dehydrogenase Animals Viability assay Cytotoxicity Tight junction Molecular biology Ochratoxins Protein Transport 030104 developmental biology chemistry Biochemistry Cell culture Toxicity Zonula Occludens-1 Protein Animal Science and Zoology DNA Damage |
| Zdroj: | Journal of animal physiology and animal nutrition. 102(1) |
| ISSN: | 1439-0396 |
| Popis: | Ochratoxin A (OTA) is a potent nephrotoxic fungi metabolite that affects animal and human health. At the cellular level, OTA is able to alter functions and viability by several mechanisms of action. Several strategies to counteract its toxicity have been studied. We investigated the role of α-tocopherol in counteracting OTA oxidative damage in Madin-Darby canine kidney (MDCK) cells by pre-incubating the cells for 3 hr with the antioxidant (1 nm, 10 μm) and then adding OTA (0-1.2 μg/ml) for the following 24 hr. Cell viability, lactate dehydrogenase (LDH) release, TUNEL staining and occludin and Zo1 localization by immunofluorescence were determined. Here, 1 nm α-tocopherol was shown to significantly reduce (p |
| Databáze: | OpenAIRE |
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