Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
| Autor: | Karine Loulier, Ignacio Arganda-Carreras, Xavier Solinas, Stephen G. Turney, Jean Livet, Solène Clavreul, Willy Supatto, Pierre Mahou, Alexis-Pierre Bemelmans, Emmanuel Beaurepaire, Jeff W. Lichtman, Katherine Matho, Anatole Chessel, Lamiae Abdeladim, Jean-Marc Sintes |
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| Přispěvatelé: | Laboratoire d'Optique et Biosciences (LOB), École polytechnique (X)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Cold Spring Harbor Laboratory (CSHL), University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU), Donostia International Physics Center - DIPC (SPAIN), Donostia International Physics Center (DIPC), University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU)-University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU), Department of Molecular and Cellular Biology, and Center for Brain Science, Harvard University, Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Harvard University [Cambridge], Arnoux, Aurélien, Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
General Physics and Astronomy 02 engineering and technology Nestin Mice Parvovirinae generation Microscopy Multiphoton excitation double markers lcsh:Science Cerebral Cortex [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics] Multidisciplinary Pyramidal Cells Resolution (electron density) Dependovirus organization 021001 nanoscience & nanotechnology Fluorescence Neuroanatomical Tract-Tracing Techniques [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs Models Animal Female 0210 nano-technology [PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics] Materials science ex-vivo Channel (digital image) fluorescent proteins Science Genetic Vectors Color Mice Transgenic Neuroimaging Transfection General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Imaging Three-Dimensional Optics Animals Humans Image acquisition Chromatic scale [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs gene axonal projections business.industry General Chemistry cell mosaic analysis Mice Inbred C57BL Luminescent Proteins HEK293 Cells Microscopy Fluorescence Multiphoton 030104 developmental biology mouse-brain Astrocytes lcsh:Q business |
| Zdroj: | Nature Communications Nature Communications, 2019, 10 (1), pp.1662. ⟨10.1038/s41467-019-09552-9⟩ Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019) Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.1662. ⟨10.1038/s41467-019-09552-9⟩ Addi. Archivo Digital para la Docencia y la Investigación instname |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-09552-9 |
| Popis: | Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and sub-micron channel registration over the entire imaged volume. We demonstrate tridimensional (3D) multicolor imaging over several cubic millimeters as well as brain-wide serial 2D multichannel imaging. We illustrate the strengths of this method through color-based 3D analysis of astrocyte morphology and contacts in the mouse cerebral cortex, tracing of individual pyramidal neurons within densely Brainbow-labeled tissue, and multiplexed whole-brain mapping of axonal projections labeled with spectrally distinct tracers. ChroMS will be an asset for multiscale and system-level studies in neuroscience and beyond. We thank Minh-Son Phan, Samuel Tozer, and Z. Josh Huang for scientific discussions and comments on the manuscript. We thank TissueVision for providing technical advice for the implementation and customization of the block-face system. We thank Bastien Binet from Polytechnique Polymedia center for his generous help in the creation of 3D graphics. We thank IdV core imaging, animal experimentation and histology facilities for technical assistance. This work was supported by fellowships from Universite Paris-Saclay (Initiatives Doctorales Interdisciplinaires) to L.A., Ecole des Neurosciences de Paris to K.M., Region Ile-de-France and Fondation ARC pour la Recherche sur le Cancer to S.C. by Agence Nationale de la Recherche under contracts ANR-11-EQPX-0029 (Equipex Morphoscope2), ANR-10-INBS-04 (France BioImaging), and ANR-10-LABX-65 (LabEx LifeSenses), by Fondation pour la Recherche Medicale (grant DBI20141231328), by Target ALS and by the European Research Council (ERC-CoG 649117). |
| Databáze: | OpenAIRE |
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