Decrease of glutamate decarboxylase (GAD)-immunoreactive nerve terminals in the substantia nigra after kainic acid lesion of the striatum
| Autor: | D.E. Schmechel, W.H. Oertel, Irwin J. Kopin, M.L. Tappaz, M J Brownstein, D.H. Ransom |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
endocrine system
medicine.medical_specialty Kainic acid Histology endocrine system diseases Carboxy-Lyases Glutamate decarboxylase Substantia nigra Striatum Immunoenzyme Techniques Lesion chemistry.chemical_compound Internal medicine medicine Animals Axon Neurons Denervation Kainic Acid Glutamate Decarboxylase nutritional and metabolic diseases Corpus Striatum Rats Substantia Nigra medicine.anatomical_structure Endocrinology nervous system chemistry Female Anatomy medicine.symptom Free nerve ending |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 29:977-980 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/29.8.7024401 |
| Popis: | Antiserum sheep 3 against rat brain glutamate decarboxylase (GAD) was employed for the immunohistochemical localization of GAD-immunoreactive nerve terminals in the substantia nigra (SN). To test whether the antiserum specifically localized GAD-containing axon terminals, the effect of kainic acid-induced striatal lesions on the reactive nerve endings in the SN was investigated. Seven days after the injection of 1 microgram kainic acid into the striatum, a 65% decrease in GAD-enzyme activity occurred in the ipsilateral SN. On immunohistochemical examination there was correspondingly a marked reduction of GAD-positive terminals. The parallel decrease in biochemical and immunohistochemical GAD activity indicates that antiserum sheep 3 can be used as a specific immunohistochemical probed for GAD-containing elements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|