Antiviral activity of curcumin-nanoemulsion associated with photodynamic therapy in vulvar cell lines transducing different variants of HPV-16

Autor: Vanesca de Souza Lino, Letícia Figueiredo Monteleoni, Paola Jocelan Scarin Provazzi, Luisa L. Villa, Fernando Lucas Primo, Paula Rahal, Natália Maria Cândido, Silvana Maria Quintana, Patrícia Pereira dos Santos Melli, Tatiana Rabachini, Antonio Claudio Tedesco, Enrique Boccardo, Camila Fernanda Amantino, Laura Sichero, Marilia Freitas Calmon, Caroline Measso do Bonfim
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), University of Bern, Center for Translational Research in Oncology- Instituto do Cancer do Estado de Sao Paulo
Rok vydání: 2020
Předmět:
Adult
curcumin and photodynamic therapy
HPV
Curcumin
Genotype
Light
Cell Survival
medicine.medical_treatment
nanoemulsion
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
Photodynamic therapy
02 engineering and technology
Antiviral Agents
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Line
Tumor
Vulvar intraepithelial neoplasia
medicine
Humans
Human papillomavirus
Human papillomavirus 16
Photosensitizing Agents
Vulvar Neoplasms
integumentary system
Papillomavirus Infections
virus diseases
Oncogene Proteins
Viral
General Medicine
021001 nanoscience & nanotechnology
medicine.disease
female genital diseases and pregnancy complications
Bioactive compound
Repressor Proteins
chemistry
Cell culture
030220 oncology & carcinogenesis
Cancer research
Nanoparticles
Emulsions
Female
0210 nano-technology
Carcinoma in Situ
Biotechnology
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 2169-141X
2169-1401
DOI: 10.1080/21691401.2020.1725023
Popis: Made available in DSpace on 2020-12-12T02:35:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Vulvar intraepithelial neoplasia (VIN) is associated with human papillomavirus (HPV) infection. Curcumin is a natural bioactive compound with antineoplastic properties. The use of nanoparticles containing curcumin could allow a better performance of this compound in therapies. So, VIN biopsies were collected and HPV DNA detection was performed by PCR, positive samples were genotyped by Restriction Fragment Length Polymorphism (RFLP) and HPV-16 variants were determined by sequencing. HPV-16 positive vulva carcinoma cells (A431) were transduced with E-P and E-350G HPV-16 E6 variants. The viability of the transduced cells treated with nanoemulsions was determined by MTT assay. Besides, apoptosis was evaluated by enzymatic activity of Caspase-3/7. The cell viability assay showed that both the empty nanoemulsion (NE-V) and the nanoemulsion of curcumin (NE-CUR) had little effect on cell viability as compared to control cells. Additionally, we observed that cells irradiated in the presence of NE-CUR presented 90% of cell death. The apoptosis assay further revealed a significant increase in the activity of caspases 3 and 7 in A431 cells expressing both HPV-16 E6 variants after treatment with NE-CUR. Finally, we submitted the HPV transduced A431 cells to organotypic cultures and observed that the combination of treatments affected tissue architecture with evident signals of tissue damage. We concluded that nanoemulsions attain good biocompatibility, since no cytotoxicity was observed and NE-CUR associated with photoactivation showed promising results, leading to death only in cells subjected to irradiation. This drug delivery system associated with photodynamic therapy may become promising in the treatment of vulva lesions. Laboratory of Genomic Studies Sao Paulo State University–UNESP Department of Microbiology Institute of Biomedical Sciences University of São Paulo–USP Institute of Pharmacology University of Bern ICESP Center for Translational Research in Oncology- Instituto do Cancer do Estado de Sao Paulo Departament of Radiology and Oncology School of Medicine Universidade de Sao Paulo–USP Department of Gynecology and Obstetrics of the Ribeirão Preto Medical School USP Ribeirão Preto Department of Bioprocess and Biotechnology Faculty of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista Julio de Mesquita Filho–UNESP Araraquara Department of Chemistry Center for Nanotechnology and Tissue Engineering Faculty of Philosophy Sciences and Languages ​​of Ribeirão Preto University of São Paulo–USP Ribeirão Preto Laboratory of Genomic Studies Sao Paulo State University–UNESP Department of Bioprocess and Biotechnology Faculty of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista Julio de Mesquita Filho–UNESP Araraquara FAPESP: 2014/02064-9
Databáze: OpenAIRE
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