Fast retrograde access to projection neuron circuits underlying vocal learning in songbirds
| Autor: | Manfred Gahr, Falk Dittrich, JC Paterna, Benjamin Ehret, Rhr Hahnloser, Roman Boehringer, Benjamin F. Grewe, Robert Kasper, M Rauch, Chihiro Mori, Rocha, Kazuo Okanoya, Ryosuke O. Tachibana, Daniel Normen Düring |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Computer science Dopaminergic Projection neuron 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure nervous system medicine Biological neural network Brainbow Vocal learning Neuron Neuroscience 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/2020.02.19.955773 |
| Popis: | SummaryUnderstanding the structure and function of neural circuits underlying speech and language is a vital step towards better treatments for diseases of these systems. Songbirds, among the few animal orders that share with humans the ability to learn vocalizations from a conspecific, have provided many insights into the neural mechanisms of vocal development. However, research into vocal learning circuits has been hindered by a lack of tools for rapid genetic targeting of specific neuron populations to meet the quick pace of developmental learning. Here, we present a new viral tool that enables fast and efficient retrograde access to projection neuron populations. In zebra finches, Bengalese finches, canaries, and mice, we demonstrate fast retrograde labeling of cortical or dopaminergic neurons. We further demonstrate the suitability of our construct for detailed morphological analysis, for in vivo imaging of calcium activity, and for multicolor brainbow labeling. |
| Databáze: | OpenAIRE |
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