Terminal differentiation in human promyelocytic leukaemic cells in the absence of DNA synthesis
| Autor: | Giovanni Rovera, Nancy E. Olashaw, Pacifico Meo |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Myeloid
HL60 Phorbol Diesters Promyelocytic leukemia protein chemistry.chemical_compound Phagocytosis hemic and lymphatic diseases medicine Humans Preleukemia Interphase Cells Cultured Multidisciplinary biology DNA synthesis Cell Differentiation DNA Cell cycle Phorbols Molecular biology In vitro medicine.anatomical_structure chemistry Leukemia Myeloid biology.protein Tetradecanoylphorbol Acetate Myeloid leukaemia |
| Zdroj: | Nature. 284:69-70 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/284069a0 |
| Popis: | Some phorbol diesters, the most potent of which is 12-O-tetradecanoylphorbol-13-acetate (TPA), exert two different effects on the differentiation of leukaemic cells in vitro: they reversibly inhibit the differentiation in Friend mouse erythroleukaemia cells1,2 and mouse myeloid leukaemia M1 cells3, and they induce differentiation in other mouse erythroleukaemia cells4, mouse myeloid leukaemia cells5, human promyelocytic leukaemia cells5–9 and cells of patients with myeloid and myelomonocytic leukaemia10. The effect on human leukaemic cells is both irreversible and independent of the continuous presence of the drug7. We report here that TPA-induced differentiation of human promyelocytic leukaemia cells (HL60) need not be preceded by a round of DNA synthesis, and that the majority of TPA-treated cells accumulate in the G1 phase of the cell cycle. |
| Databáze: | OpenAIRE |
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