Does supplementation with leucine-enriched protein alone and in combination with fish-oil-derived n-3 PUFA affect muscle mass, strength, physical performance, and muscle protein synthesis in well-nourished older adults? A randomized, double-blind, placebo-controlled trial
| Autor: | Davide Susta, Ricardo Segurado, Kathleen A. J. Mitchelson, Joan M. G. Senden, Caoileann H. Murphy, Giuseppe De Vito, Ellen M Flanagan, Sinead McCarthy, Brendan Egan, Joy P B Goessens, Adrian Chabowski, Luc J. C. van Loon, Clare A. Corish, Helen M. Roche, Elena de Marco Castro, Agnieszka Mikłosz |
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| Přispěvatelé: | Humane Biologie, RS: NUTRIM - R3 - Respiratory & Age-related Health, Physiotherapy, Human Physiology and Anatomy, Human Physiology and Sports Physiotherapy Research Group |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Muscle Proteins Medicine (miscellaneous) Gene Expression Regulation/drug effects Isometric exercise Muscle Strength/drug effects AMINO-ACID SUPPLEMENTATION AcademicSubjects/MED00160 DIETARY-FAT MODIFICATION VITAMIN-D chemistry.chemical_classification Muscle Proteins/genetics Nutrition and Dietetics biology LC n-3 PUFA Physical Functional Performance Fish oil HEALTHY OLDER Fatty Acids Omega-3/administration & dosage Original Research Communications muscle mass Body Composition Female Muscle Skeletal/drug effects aging LC n–3 PUFA leucine protein muscle mass strength sarcopenia leucine strength Polyunsaturated fatty acid medicine.medical_specialty BODY-COMPOSITION LC n–3 PUFA Renal function Nutritional Status EXERCISE ELDERLY-PEOPLE sarcopenia AcademicSubjects/MED00060 AGE Double-Blind Method Internal medicine Fatty Acids Omega-3 medicine Humans Muscle Strength Muscle Skeletal Aged Adiponectin business.industry SERUM CYSTATIN-C aging biomarkers medicine.disease Endocrinology Gene Expression Regulation chemistry Cystatin C Sarcopenia biology.protein Lean body mass RISK-FACTORS business aged 80 and over protein |
| Zdroj: | Murphy, C H, Flanagan, E M, De Vito, G, Susta, D, Mitchelson, K A J, de Marco Castro, E, Senden, J M G, Goessens, J P B, Mikłosz, A, Chabowski, A, Segurado, R, Corish, C A, McCarthy, S N, Egan, B, van Loon, L J C & Roche, H M 2021, ' Does supplementation with leucine-enriched protein alone and in combination with fish-oil-derived n-3 PUFA affect muscle mass, strength, physical performance, and muscle protein synthesis in well-nourished older adults? A randomized, double-blind, placebo-controlled trial ', The American journal of clinical nutrition, vol. 113, no. 6, pp. 1411–1427 . https://doi.org/10.1093/ajcn/nqaa449 American Journal of Clinical Nutrition, 113(6), 1411-1427. Oxford University Press The American Journal of Clinical Nutrition |
| ISSN: | 0002-9165 |
| DOI: | 10.1093/ajcn/nqaa449 |
| Popis: | Background: Leucine-enriched protein (LEU-PRO) and long-chain(LC) n–3 (ω–3) PUFAs have each been proposed to improve muscle mass and function in older adults, whereas their combination may be more effective than either alone.Objective: The impact of LEU-PRO supplementation alone and combined with LC n–3 PUFAs on appendicular lean mass, strength, physical performance and myofibrillar protein synthesis(MyoPS) was investigated in older adults at risk of sarcopenia.Methods: This 24-wk, 3-arm parallel, randomized, double-blind, placebo-controlled trial was conducted in 107 men and women aged ≥65 y with low muscle mass and/or strength. Twice daily, participants consumed a supplement containing either LEU-PRO(3 g leucine, 10 g protein; n = 38), LEU-PRO plus LC n–3 PUFAs (0.8 g EPA, 1.1 g DHA; LEU-PRO+n–3; n = 38),or an isoenergetic control (CON; n = 31). Appendicular lean mass, handgrip strength, leg strength, physical performance, and circulating metabolic and renal function markers were measured pre-, mid-, and postintervention. Integrated rates of MyoPS were assessed in a subcohort (n = 28).Results: Neither LEU-PRO nor LEU-PRO+n–3 supplementation affected appendicular lean mass, handgrip strength, knee extension strength, physical performance or MyoPS. However, isometric knee flexion peak torque (treatment effect: −7.1 Nm; 95% CI: −12.5,−1.8 Nm; P < 0.01) was lower post supplementation in LEUPRO+n–3 compared with CON. Serum triacylglycerol and total adiponectin concentrations were lower, and HOMA-IR was higher, in LEU-PRO+n–3 compared with CON postsupplementation (allP < 0.05). Estimated glomerular filtration rate was higher and cystatin c was lower in LEU-PRO and LEU-PRO+n–3 postsupplementation compared with CON (all P < 0.05).Conclusions: Contrary to our hypothesis, we did not observe a beneficial effect of LEU-PRO supplementation alone or combined with LC n–3 PUFA supplementation on appendicular lean mass, strength, physical performance or MyoPS in older adults at risk of sarcopenia. This trial was registered at clinicaltrials.gov asNCT03429491. |
| Databáze: | OpenAIRE |
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