Performance of Paracoccus pantotrophus MA3 in heterotrophic nitrification–anaerobic denitrification using formic acid as a carbon source
Autor: | Qun Huang, Ahmed Alengebawy, Xiangyu Zhu, Amin Farrukh Raza, Limei Chen, Wuxi Chen, Jiahao Guo, Ping Ai, Demao Li |
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Rok vydání: | 2022 |
Předmět: |
Glycine Hydroxymethyltransferase
Methylenetetrahydrofolate Dehydrogenase (NADP) Nitrates Formates Nitrogen Glutamate Synthase Water Bioengineering General Medicine Wastewater Formate Dehydrogenases Nitrate Reductase Nitrification Aerobiosis Carbon Formate-Tetrahydrofolate Ligase Glutamate Dehydrogenase Glutamate-Ammonia Ligase Paracoccus pantotrophus Biofuels Ammonium Compounds Denitrification Anaerobiosis Biotechnology |
Zdroj: | Bioprocess and Biosystems Engineering. 45:1661-1672 |
ISSN: | 1615-7605 1615-7591 |
Popis: | Excess amount of nitrogen in wastewater has caused serious concerns, such as water eutrophication. Paracoccus pantotrophus MA3, a novel isolated strain of heterotrophic nitrification-anaerobic denitrification bacteria, was evaluated for nitrogen removal using formic acid as the sole carbon source. The results showed that the maximum ammonium removal efficiency was observed under the optimum conditions of 26.25 carbon to nitrogen ratio, 3.39% (v/v) inoculation amount, 34.64 °C temperature, and at 180 rpm shaking speed, respectively. In addition, quantitative real-time PCR technique analysis assured that the gene expression level of formate dehydrogenase, formate tetrahydrofolate ligase, 5,10-methylenetetrahydrofolate dehydrogenase, serine hydroxymethyltransferase, respiratory nitrate reductase beta subunit, L-glutamine synthetase, glutamate dehydrogenase, and glutamate synthase were up-regulated compared to the control group, and combined with nitrogen mass balance analysis to conclude that most of the ammonium was removed by assimilation. A small amount of nitrate and nearly no nitrite were accumulated during heterotrophic nitrification. MA3 exhibited significant denitrification potential under anaerobic conditions with a maximum nitrate removal rate of 4.39 mg/L/h, and the only gas produced was N |
Databáze: | OpenAIRE |
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