Studies on testosterone-17β-glucosiduronate in human plasma
| Autor: | J. A. F. Tresguerres, J. Tamm, U. Volkwein |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Adult
Male Hirsutism medicine.medical_specialty Testicular vein Coefficient of variation Radioimmunoassay Glucuronates Chorionic Gonadotropin Biochemistry Significant elevation Endocrinology Reference Values Internal medicine medicine Humans Testosterone Chemistry Hypogonadism Specific radioimmunoassay medicine.vein Human plasma Plasma concentration Female Steady state (chemistry) |
| Zdroj: | Journal of Steroid Biochemistry. 8:1071-1075 |
| ISSN: | 0022-4731 |
| DOI: | 10.1016/0022-4731(77)90276-x |
| Popis: | A specific radioimmunoassay (RIA) for the determination of testosterone-17β-glucosiduronate (TG) in human plasma is described. A sensitivity of 25 pg was obtained in the standard curve with an intra-assay coefficient of variation (CV) of 8.5% and an inter-assay CV of 12.5%. Plasma TG concentrations were found as follows (mean ± S.D., ng/ml): Normal males 2.1 ± 0.45 ( n = 20); normal females 0.47 ± 0.18 ( n = 17); hirsute females 1.36 ± 0.68 ( n = 14); hypogonadic males 1.08 ± 0.3 ( n = 10). TG concentration in the testicular vein of a normal male was 1.91 ng/ml, 30 min after 5000 IU HCG iv 3.0 ng/ml. The infusion of 2mg of TG for 2 h to normal men induced a 16-fotd increase in plasma TG levels, reaching a steady state after 1 h. Plasma concentrations of unconjugated testosterone (T) and 5α-dihydrotestosterone (DHT) estimated simultaneously were not altered. Intravenous infusions of 1000 IU HCG caused a significant elevation of plasma T but no change in plasma TG levels. |
| Databáze: | OpenAIRE |
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