Double-Stranded DNA Fragments Bearing Unrepairable Lesions and Their Internalization into Mouse Krebs-2 Carcinoma Cells
| Autor: | Sergey S. Bogachev, Olga I. Lavrik, Ekaterina A. Potter, Alexey A. Popov, I. O. Petruseva, A. N. Evdokimov, Anastasia S. Proskurina, E. V. Dolgova, Sergey I. Bayborodin, Yaroslav R. Efremov |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DNA Repair health care facilities manpower and services media_common.quotation_subject education DNA Fragmentation Biochemistry 03 medical and health sciences chemistry.chemical_compound DNA Adducts Mice 0302 clinical medicine Cell Line Tumor Drug Discovery Genetics Extracellular Animals Humans Internalization Molecular Biology health care economics and organizations media_common Chemistry Carcinoma DNA Cell biology Krebs 2 Carcinoma 030104 developmental biology 030220 oncology & carcinogenesis Molecular Medicine Stem cell Double stranded |
| Zdroj: | Nucleic acid therapeutics. 29(5) |
| ISSN: | 2159-3345 |
| Popis: | Murine Krebs-2 tumor-initiating stem cells are known to natively internalize extracellular double-stranded DNA fragments. Being internalized, these fragments interfere in the repair of chemically induced interstrand cross-links. In the current investigation, 756 bp polymerase chain reaction (PCR) product containing bulky photoreactive dC adduct was used as extracellular DNA. This adduct was shown to inhibit the cellular system of nucleotide excision repair while being resistant to excision by this DNA repair system. The basic parameters for this DNA probe internalization by the murine Krebs-2 tumor cells were characterized. Being incubated under regular conditions (60 min, 24°C, 500 μL of the incubation medium, in the dark), 0.35% ± 0.18% of the Krebs-2 ascites cells were shown to natively internalize modified DNA. The saturating amount of the modified DNA was detected to be 0.37 μg per 10 |
| Databáze: | OpenAIRE |
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