Graphene oxide@gold nanorods-based multiple-assisted electrochemiluminescence signal amplification strategy for sensitive detection of prostate specific antigen
| Autor: | Jun-Tao Cao, Yan-Ming Liu, Yu-Ling Wang, Jiu-Jun Yang, Shu-Hui Ma, Hui Wang, Li-Zhen Zhao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Streptavidin
endocrine system Aptamer Biomedical Engineering Biophysics Biotin Metal Nanoparticles Nanotechnology Biosensing Techniques 02 engineering and technology 01 natural sciences Signal Luminol Glucose Oxidase chemistry.chemical_compound Limit of Detection Electrochemistry Humans Electrochemiluminescence Glucose oxidase Detection limit Nanotubes biology 010401 analytical chemistry Hydrogen Peroxide General Medicine Aptamers Nucleotide Prostate-Specific Antigen 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences chemistry Colloidal gold biology.protein Graphite Gold 0210 nano-technology Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 99:92-98 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2017.07.050 |
| Popis: | A novel and competitive electrochemiluminescence (ECL) aptasensor for prostate specific antigen (PSA) assay was constructed using gold nanorods functionalized graphene oxide (GO@AuNRs) multilabeled with glucose oxidase (GOD) and streptavidin (SA) toward luminol-based ECL system. A strong initial ECL signal was achieved by electrodeposited gold (DpAu) on the electrode because of gold nanoparticles (AuNPs) motivating the luminol ECL signal. The signal probes prepared by loading GOD and SA-biotin-DNA on GO@AuNRs were used for achieving multiple signal amplification. In the absence of PSA, the signal probes can be attached on the electrode by hybridization reaction between PSA aptamer and biotin-DNA. In this state, the GOD loaded on the probe could catalyze glucose to in situ produce H2O2 and then AuNRs catalyze H2O2 to generate abundant reactive oxygen species (ROSs) in luminol ECL reaction. Both the high-content GOD and AuNRs in the signal probe amplified the ECL signal in the ECL system. Moreover, the combination of SA with biotin-DNA further expands ECL intensity. The integration of such amplifying effects in this protocol endows the aptasensor with high sensitivity and good selectivity for PSA detection. This aptasensor exhibits a linear relation in the range of 0.5pgmL-1 to 5.0ngmL-1 with the detection limit of 0.17pgmL-1 (S/N = 3). Besides, the strategy was successfully applied in determination of human serum samples with recovery of 81.4-116.0%. |
| Databáze: | OpenAIRE |
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