Overexpression of PMA1 enhances tolerance to various types of stress and constitutively activates the SAPK pathways in Saccharomyces cerevisiae
| Autor: | Eun Jung Kim, Young-Mi Lee, Yeji Lee, Wankee Kim, Wonja Choi, Olviyani Nasution |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Saccharomyces cerevisiae Proteins MAP kinase kinase kinase biology Membrane permeability Kinase Saccharomyces cerevisiae Gene Expression General Medicine biology.organism_classification Applied Microbiology and Biotechnology Cell biology 03 medical and health sciences Proton-Translocating ATPases 030104 developmental biology Biochemistry Stress Physiological Cyclic AMP Response Element Mitogen-Activated Protein Kinases Protein kinase A Transcription factor Biotechnology Signal Transduction |
| Zdroj: | Applied microbiology and biotechnology. 101(1) |
| ISSN: | 1432-0614 |
| Popis: | PMA1 encodes a transmembrane polypeptide that functions to pump protons out of the cell. Ectopic PMA1 overexpression in Saccharomyces cerevisiae enhances tolerance to weak acids, reactive oxygen species (ROS) and ethanol, and changes the following physiological properties: better proton efflux, lower membrane permeability, and lessened internal hydrogen peroxide production. The enhanced stress tolerance was dependent on the mitogen-activated protein kinase (MAPK) Hog1 of the high osmolarity glycerol (HOG) pathway, but not the MAPK Slt2 of the cell wall integrity (CWI) pathway; however, a PMA1 overexpression constitutively activated both Hog1 and Slt2. The constitutive Hog1 activation required the MAPK kinase kinase (MAP3K) Ssk2 of the HOG pathway, but not Ste11 and Ssk22, two other MAP3Ks of the same pathway. The constitutive Slt2 activation did not require Rom2 and the membrane sensors of the CWI pathway, whereas Bck1 was indispensable. The PMA1 overexpression activated the stress response element but not the cyclic AMP response element and the Rlm1 transcription factor. PMA1 overexpression may facilitate the construction of industrial strains with simultaneous tolerance to weak acids, ROS, and ethanol. |
| Databáze: | OpenAIRE |
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