Analysis of illicit amphetamine seizures by capillary zone electrophoresis
| Autor: | Frans Parmentier, Véronique Piette |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Capillary action
Analytical chemistry Electrolyte Buffers Biochemistry Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Spectrophotometry medicine Ephedrine Phosphoric acid Chromatography medicine.diagnostic_test Chemistry Illicit Drugs Organic Chemistry Amphetamines Electrophoresis Capillary Reproducibility of Results General Medicine Hydrogen-Ion Concentration Electrophoresis Triethanolamine Spectrophotometry Ultraviolet medicine.drug |
| Zdroj: | Journal of chromatography. A. 979(1-2) |
| ISSN: | 0021-9673 |
| Popis: | Capillary zone electrophoresis was applied for the determination of amphetamine and related substances in seized drugs. A buffer made of 0.1 M phosphoric acid adjusted to pH 3.0 with triethanolamine was selected. With this background electrolyte, triethanolamine is adsorbed to the capillary wall and the electroosmotic flow is reversed. This gives rise to peaks with good symmetry, high efficiency and reproducible migration times. The separation of the different analytes was performed in a fused-silica capillary thermostatted at 25 degrees C and the applied voltage was 25 kV. Under these experimental conditions, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethamphetamine, N-methyl-1-(1,3-benzodioxol-5-yl)-2-butamine and ephedrine were resolved within 8 min and without interference from adulterants usually found in illicit powders. Their identification by the migration time was confirmed by their UV spectra recorded with a diode array UV detector (190-350 nm). The selected method was then applied to identify these substances in illicit tablets known as "Ecstasy" and the MDMA determined in these samples according to a laboratory validation procedure. |
| Databáze: | OpenAIRE |
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