Induction of Duffy gene (FY) in human endothelial cells and in mouse
| Autor: | Hong Luo, A. Oscar Pogo, Asok Chaudhuri, Marilis Rodriguez, V. Zbrzezna, Deben Banerjee |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Lipopolysaccharides
Chemokine Umbilical Veins Time Factors Endothelium Immunology Chemokine CXCL2 Inflammation Receptors Cell Surface Cell Separation Pulmonary Artery Biochemistry Proinflammatory cytokine Mice Antigen Gene expression medicine Immunology and Allergy Animals Humans RNA Messenger Cycloheximide Molecular Biology Lung Cells Cultured Protein Synthesis Inhibitors biology Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Monokines Brain Hematology Molecular biology Immunohistochemistry Blot Mice Inbred C57BL medicine.anatomical_structure Microscopy Fluorescence biology.protein Tumor necrosis factor alpha Endothelium Vascular medicine.symptom Duffy Blood-Group System |
| Zdroj: | Cytokine. 21(3) |
| ISSN: | 1043-4666 |
| Popis: | Duffy Blood Group protein is a glycoprotein with seven transmembrane domains that binds to C-X-C and C-C chemokines. The antigen is constitutively expressed in endothelial and epithelial cells of several nonerythroid tissues and in Purkinje cells of the cerebellum. We studied the effect of proinflammatory cytokines on Duffy gene expression in endothelial cells from human umbilical vein (HUVEC) and human pulmonary arteries (HPAEC). Also, we studied the effect of inflammatory agents like bacterial lipopolysaccharide (LPS) on Duffy gene induction in mouse. Reverse transcription-PCR and mRNA blot analyses showed that Duffy mRNA was present in these cells in negligible amounts. However, treatment with tumor necrosis factor-alpha for 6-24h resulted in a 5 to 8-fold increase in Duffy mRNA. On the other hand, treatment with interleukin-1 (IL-1), IL-6 or LPS did not have any effect. Fluorescence microscopy and fluorescence activated cell sorting showed greater expression of Duffy protein in treated cells correlating the increase in mRNA synthesis with an increase in antigen production. In mice, Duffy gene was induced in lungs and brain with LPS treatment indicating that the induction is a physiological event. Vascular endothelial cells may induce Duffy protein to regulate leukocytes and/or chemokine trafficking. |
| Databáze: | OpenAIRE |
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