Deletions on mouse Yq lead to upregulation of multiple X- and Y-linked transcripts in spermatids
| Autor: | Lydia Ferguson, Peter J.I. Ellis, Aminata Touré, Penelope Alexandra Falshaw Ball, Kate L Loveland, Paul S. Burgoyne, Nabeel A. Affara, James M. A. Turner, Emily J Clemente |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Biology Y chromosome Mice Intragenomic conflict Genes Y-Linked Genes X-Linked X Chromosome Inactivation Y Chromosome Genetics Animals Sex Ratio Molecular Biology Gene Psychological repression Genetics (clinical) X-linked recessive inheritance X chromosome In Situ Hybridization Adaptor Proteins Signal Transducing Regulation of gene expression Reverse Transcriptase Polymerase Chain Reaction Nuclear Proteins General Medicine Blotting Northern Microarray Analysis Spermatids Adaptor Proteins Vesicular Transport Blotting Southern Gene Expression Regulation Multigene Family Y linkage Gene Deletion |
| Zdroj: | Human molecular genetics. 14(18) |
| ISSN: | 0964-6906 |
| Popis: | Deletions on the mouse Y-chromosome long arm (MSYq) lead to teratozoospermia and in severe cases to infertility. We find that the downstream transcriptional changes in the testis resulting from the loss of MSYq-encoded transcripts involve upregulation of multiple X- and Y-linked spermatid-expressed genes, but not related autosomal genes. Therefore, this indicates that in normal males, there is a specific repression of X and Y (gonosomal) transcription in post-meiotic cells, which depends on MSYq-encoded transcripts. Together with the known sex ratio skew in favour of females in the offspring of fertile MSYqdel males, this strongly suggests the existence of an intragenomic conflict between X- and Y-linked genes. Two potential antagonists in this conflict are the X-linked multicopy gene Xmr and its multicopy MSYq-linked relative Sly, which are upregulated and downregulated, respectively, in the testes of MSYqdel males. Xmr is also expressed during meiotic sex chromosome inactivation (MSCI), indicating a link between the MSCI and the MSYq-dependent gonosomal repression in spermatids. We therefore propose that this repression and MSCI itself are evolutionary adaptations to maintain a normal sex ratio in the face of X/Y antagonism. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|