Fabrication and practical applications of molybdenum disulfide nanopores
| Autor: | Juraj Topolancik, Mukeshchand Thakur, Ke Liu, Jiandong Feng, Martina Lihter, Vasileia Georgiou, Yann Astier, B. Robert Ilic, Michael Graf, Aleksandra Radenovic |
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| Předmět: |
chemistry.chemical_classification
0303 health sciences Fabrication Materials science Biomolecule Nanotechnology Solid-state nanopore nanopore 2D-material molybdenum disulfide MoS2 transition metal dichalcogenide fabrication PMMA PDMS transfer single-molecule detection osmotic power generation reverse electrodialysis transmission electron microscopy drilling electrochemical reaction ECR nanopore analysis translocation General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Nanopore 0302 clinical medicine Membrane chemistry Reversed electrodialysis Monolayer Osmotic power Molybdenum disulfide 030217 neurology & neurosurgery 030304 developmental biology |
| Popis: | Among the different developed solid-state nanopores, nanopores constructed in a monolayer of molybdenum disulfide (MoS2) stand out as powerful devices for single-molecule analysis or osmotic power generation. Because the ionic current through a nanopore is inversely proportional to the thickness of the pore, ultrathin membranes have the advantage of providing relatively high ionic currents at very small pore sizes. This increases the signal generated during translocation of biomolecules and improves the nanopores' efficiency when used for desalination or reverse electrodialysis applications. The atomic thickness of MoS2 nanopores approaches the inter-base distance of DNA, creating a potential candidate for DNA sequencing. In terms of geometry, MoS2 nanopores have a well-defined vertical profile due to their atomic thickness, which eliminates any unwanted effects associated with uneven pore profiles observed in other materials. This protocol details all the necessary procedures for the fabrication of solid-state devices. We discuss different methods for transfer of monolayer MoS2, different approaches for the creation of nanopores, their applicability in detecting DNA translocations and the analysis of translocation data through open-source programming packages. We present anticipated results through the application of our nanopores in DNA translocations and osmotic power generation. The procedure comprises four parts: fabrication of devices (2-3 d), transfer of MoS2 and cleaning procedure (24 h), the creation of nanopores within MoS2 (30 min) and performing DNA translocations (2-3 h). We anticipate that our protocol will enable large-scale manufacturing of single-molecule-analysis devices as well as next-generation DNA sequencing. |
| Databáze: | OpenAIRE |
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