GLAD-PCR Assay of R(5mC)GY Sites in the Regulatory Region of Tumor-Suppressor Genes Associated with Gastric Cancer
| Autor: | Malyshev, B. S., Netesova, N. A., Smetannikova, N. A., Abdurashitov, M. A., Akishev, A. G., Dubinin, E. V., Azanov, A. Z., Vihlyanov, I. V., Nikitin, M. K., Karpov, A. B., Degtyarev, S. Kh. |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
IRX1 Biology medicine.disease_cause Biochemistry DNA methyltransferase methyl-directed DNA endonuclease GlaI 03 medical and health sciences 0302 clinical medicine medicine Epigenetics Gene Molecular Biology DNA methylation gastric cancer Cancer GLAD-PCR assay medicine.disease Molecular biology 030104 developmental biology Regulatory sequence 030220 oncology & carcinogenesis Molecular Medicine tumor suppressor genes Carcinogenesis Biotechnology Research Article |
| Zdroj: | Acta Naturae |
| ISSN: | 2075-8251 |
| Popis: | At early stages of carcinogenesis, the regulatory regions of some tumor suppressor genes become aberrantly methylated at RCGY sites, which are substrates of DNA methyltransferase Dnmt3. Identification of aberrantly methylated sites in tumor DNA is considered to be the first step in the development of epigenetic PCR test systems for early diagnosis of cancer. Recently, we have developed a GLAD-PCR assay, a method for detecting the R(5mC)GY site in the genome position of interest even at significant excess of DNA molecules with a non-methylated RCGY site in this location. The aim of the present work is to use the GLAD-PCR assay to detect the aberrantly methylated R(5mC)GY sites in the regulatory regions of tumor suppressor genes (brinp1, bves, cacna2d3, cdh11, cpeb1, epha7, fgf2, galr1, gata4, hopx, hs3st2, irx1, lrrc3b, pcdh10, rprm, runx3, sfrp2, sox17, tcf21, tfpi2, wnt5a, zfp82, and znf331) in DNA samples obtained from gastric cancer (GC) tissues. The study of the DNA samples derived from 29 tumor and 25 normal gastric tissue samples demonstrated a high diagnostic potential of the selected RCGY sites in the regulatory regions of the irx1, cacna2d3, and epha7 genes; the total indices of sensitivity and specificity for GC detection being 96.6% and 100%, respectively. |
| Databáze: | OpenAIRE |
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