Discovery and validation of candidate genes for grain iron and zinc metabolism in pearl millet [Pennisetum glaucum (L.) R. Br.]
| Autor: | Rakesh K. Srivastava, Mahesh D. Mahendrakar, Maheshwari Parveda, P. B. Kavi Kishor |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Candidate gene Pennisetum Iron chemistry.chemical_element lcsh:Medicine Zinc Biology Quantitative trait locus Genes Plant 01 natural sciences Article 03 medical and health sciences Gene expression Genetics Gene family Sequencing Nutritional Physiological Phenomena lcsh:Science Gene Genetic Association Studies Plant Physiological Phenomena Plant Proteins Molecular breeding Multidisciplinary lcsh:R food and beverages DNA Shuffling Genomics biology.organism_classification 030104 developmental biology chemistry lcsh:Q Plant sciences 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Pearl millet is an important crop for alleviating micronutrient malnutrition through genomics-assisted breeding for grain Fe (GFeC) and Zn (GZnC) content. In this study, we identified candidate genes related to iron (Fe) and zinc (Zn) metabolism through gene expression analysis and correlated it with known QTL regions for GFeC/GZnC. From a total of 114 Fe and Zn metabolism-related genes that were selected from the related crop species, we studied 29 genes. Different developmental stages exhibited tissue and stage-specific expressions for Fe and Zn metabolism genes in parents contrasting for GFeC and GZnC. Results revealed that PglZIP, PglNRAMP and PglFER gene families were candidates for GFeC and GZnC. Ferritin-like gene, PglFER1 may be the potential candidate gene for GFeC. Promoter analysis revealed Fe and Zn deficiency, hormone, metal-responsive, and salt-regulated elements. Genomic regions underlying GFeC and GZnC were validated by annotating major QTL regions for grain Fe and Zn. Interestingly, PglZIP and PglNRAMP gene families were found common with a previously reported linkage group 7 major QTL region for GFeC and GZnC. The study provides insights into the foundation for functional dissection of different Fe and Zn metabolism genes homologs and their subsequent use in pearl millet molecular breeding programs globally. |
| Databáze: | OpenAIRE |
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