ZmMYC2 exhibits diverse functions and enhances JA signaling in transgenic Arabidopsis

Autor: Lijun Liu, Qin Liu, Panpan Yang, Qinqin Shen, Chenying Zhu, Qiang Wang, Jingye Fu, Chang Wang
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Transgene
Arabidopsis
Plant Science
Cyclopentanes
Biology
01 natural sciences
Zea mays
Anthocyanins
03 medical and health sciences
Plant Growth Regulators
Auxin
Gene Expression Regulation
Plant
Sequence Analysis
Protein
Two-Hybrid System Techniques
Gene expression
Jasmonate
Oxylipins
Transcription factor
Disease Resistance
Plant Diseases
chemistry.chemical_classification
Arabidopsis Proteins
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
food and beverages
Promoter
General Medicine
biology.organism_classification
Plants
Genetically Modified
Cell biology
030104 developmental biology
chemistry
Plant hormone
Botrytis
Transcriptome
Agronomy and Crop Science
Sequence Alignment
010606 plant biology & botany
Signal Transduction
Transcription Factors
Zdroj: Plant cell reports. 39(2)
ISSN: 1432-203X
Popis: ZmMYC2 was identified as the key regulator of JA signaling in maize and exhibited diverse functions through binding to many gene promoters as well as enhanced JA signaling in transgenic Arabidopsis. The plant hormone jasmonate (JA) extensively coordinates plant growth, development and defensive responses. MYC2 is the master regulator of JA signaling and has been widely studied in many plant species. However, little is known about this transcription factor in maize. Here, we identified one maize transcription factor with amino acid identity of 47% to the well-studied Arabidopsis AtMYC2, named as ZmMYC2. Gene expression analysis demonstrated inducible expression patterns of ZmMYC2 in response to multiple plant hormone treatments, as well as biotic and abiotic stresses. The yeast two-hybrid assay indicated physical interaction among ZmMYC2 and JA signal repressors ZmJAZ14, ZmJAZ17, AtJAZ1 and AtJAZ9. ZmMYC2 overexpression in Arabidopsis myc2myc3myc4 restored the sensitivity to JA treatment, resulting in shorter root growth and inducible anthocyanin accumulation. Furthermore, overexpression of ZmMYC2 in Arabidopsis elevated resistance to Botrytis cinerea. Further ChIP-Seq analysis revealed diverse regulatory roles of ZmMYC2 in maize, especially in the signaling crosstalk between JA and auxin. Hence, we identified ZmMYC2 and characterized its roles in regulating JA-mediated growth, development and defense responses.
Databáze: OpenAIRE
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