MaGiK Method of T-Cell Receptor Repertoire Analysis
| Autor: | M. Scott Killian, Janis V. Giorgi, Roger Detels, Jose L. Matud, Beth D. Jamieson |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Microbiology (medical) Magik Statistics as Topic Clinical Biochemistry Immunology Receptors Antigen T-Cell Complementarity determining region Computational biology Common method Biology Polymerase Chain Reaction Cdr3 region Antigen T-Lymphocyte Subsets Receptor repertoire Cellular Immunology Humans Immunology and Allergy Gene Rearrangement beta-Chain T-Cell Antigen Receptor computer.programming_language Genetics Middle Aged Complementarity Determining Regions Clone Cells T-Cell Receptor Repertoire Molecular Diagnostic Techniques Female Identification (biology) computer |
| Zdroj: | Clinical and Vaccine Immunology. 9:858-863 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cdli.9.4.858-863.2002 |
| Popis: | T-cell receptor diversity enables the cellular immune response to recognize a broad range of viral and other pathogenic agents. An increasingly common method of characterizing T-cell receptor diversity and usage in response to antigenic challenges involves the identification of clonal expansions by PCR amplification of the CDR3 region of distinct TCRVβ families. Though clonal expansions often appear evident upon visual inspection of the results, a systematic method is needed for the valid enumeration of these expansions. Here, we describe a novel analysis method, termed the MaGiK method, for systematically identifying and enumerating clonal T-cell expansions and for applying the results to investigations of the T-cell receptor repertoire. |
| Databáze: | OpenAIRE |
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