Sensitive determination of nitrotyrosine in human plasma by isocratic high-performance liquid chromatography
| Autor: | Yosuke Kishimoto, Yoshinori Kamisaki, Masayuki Kitano, Kentaro Nakamoto, Tadao Itoh, Kouichirou Wada |
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| Rok vydání: | 1996 |
| Předmět: |
Detection limit
Chromatography Coefficient of variation Nitrotyrosine Reproducibility of Results General Chemistry Sensitivity and Specificity High-performance liquid chromatography Nitric oxide chemistry.chemical_compound 4-Chloro-7-nitrobenzofurazan chemistry Blood plasma Humans Tyrosine Quantitative analysis (chemistry) Biomarkers Chromatography High Pressure Liquid Peroxynitrite Fluorescent Dyes |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 685:343-347 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(96)00202-2 |
| Popis: | A highly sensitive and simple isocratic high-performance liquid chromatographic method was developed for determination of 3-nitrotyrosine in human plasma with precolumn derivatization with 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole. The precision of the method was satisfactory (coefficient of variation 4.8%), and the detection limit was established at 0.1 pmol of 3-nitrotyrosine allowing the determination at the level of 6 pmol/ml in human plasma. The recoveries of 3-nitrotyrosine and alpha-methyltyrosine, an internal standard, were 89.3 +/- 7.1 and 85.7 +/- 7.6%, respectively. The 3-nitrotyrosine level was 31 +/- 6 pmol/ml (mean +/- S.D., n = 9) in plasma from healthy volunteers. Since 3-nitrotyrosine is a stable product of peroxynitrite, an oxidant formed by a reaction of nitric oxide and superoxide radicals, the measurement of its plasma concentration may be useful as a marker of nitric oxide-dependent oxidative damage. |
| Databáze: | OpenAIRE |
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