Co-administration of toll-like receptor (TLR)-3 and 4 ligands augments immune response to Newcastle disease virus (NDV) vaccine in chicken
| Autor: | E. Priyanka, Maddula R. Reddy, T R Kannaki |
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| Rok vydání: | 2018 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide animal diseases medicine.medical_treatment Newcastle Disease Newcastle disease virus chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Biology Newcastle disease Virus chemistry.chemical_compound Immune system Adjuvants Immunologic medicine Animals Toll-like receptor Immunity Cellular General Veterinary Viral Vaccines General Medicine biochemical phenomena metabolism and nutrition Acquired immune system biology.organism_classification Immunity Humoral Toll-Like Receptor 3 Toll-Like Receptor 4 Poly I-C chemistry Immunology TLR4 Adjuvant Chickens |
| Zdroj: | Veterinary research communications. 43(4) |
| ISSN: | 1573-7446 |
| Popis: | Toll-like receptors (TLRs) are pattern recognition receptors (PRRs) that mediate first line of host defence to pathogens. TLR agonists are potent immunostimulatory agents that help to prime a robust adaptive immune response. In the present study, adjuvant potential of Poly I:C and lipopolysaccharide (LPS) were evaluated with live Newcastle disease virus (NDV) vaccine. Cornish chickens were immunized with live Newcastle disease virus (NDV) vaccine (R2B-mesogenic strain) adjuvanted either with Poly I:C (TLR3 agonist) or LPS-TLR4 agonist and both. Humoral Immune response to ND vaccine was evaluated through haemagglutination inhibition (HI) test and ELISA, while the cellular immune response (CMI) was quantified by lymphocyte transformation test (LTT). IL-1β cytokine mRNA levels in spleen tissue were also quantified by real time PCR. The results suggest that TLR3 and TLR4 agonists are an efficient immune-stimulators separately, as LPS co-administered group has shown significantly higher serum titre on second week post-immunization and Poly I:C group on third week post-immunization both by HI and ELISA (P |
| Databáze: | OpenAIRE |
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