Cytokine gene expression profiles in chicken spleen and intestinal tissues during Ascaridia galli infection
| Autor: | Helle R. Juul-Madsen, Anders Permin, A Janne Pleidrup, Liselotte R. Norup, Dorte Fink Vadekær, Gregers Jungersen, Peter K. Kaiser, Tina S. Dalgaard, Poul Sørensen, Torben Wilde Schou |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
chicken
medicine.medical_treatment Spleen Biology Ascaridia immune response Andrology Immune system Intestinal mucosa Gene expression medicine Animals Ascaridia galli Poultry Diseases Innate immune system General Veterinary A.galli General Medicine Acquired immune system biology.organism_classification cytokines Intestines medicine.anatomical_structure Cytokine Immunology Cytokines Female Parasitology Transcriptome Chickens |
| Zdroj: | Andersen, J P, Norup, L R, Dalgaard, T S, Kaiser, P, Permin, A, Schou, T W, Vadekær, D F, Jungersen, G, Sørensen, P & Juul-Madsen, H R 2014, ' Cytokine gene expression profiles in chicken spleen and intestinal tissues during Ascaridia galli infection ', Veterinary Parasitology, vol. 206, no. 3-4, pp. 317-321 . https://doi.org/10.1016/j.vetpar.2014.10.016 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2014.10.016 |
| Popis: | In the poultry production industry, chickens with access to outdoor areas are exposed to a wide range of parasites e.g. the helminth Ascaridia galli. By real-time quantitative RT-PCR, the relative gene expression of the T helper 1 (Th1) cytokine IFN-γ, the T helper 2 (Th2) cytokine IL-13, the anti-inflammatory cytokines IL-10 and TGF-β4 and the pro-inflammatory cytokine IL-17F were determined over a period of 3 weeks in A. galli and non-A. galli-infected chickens. A characteristic Th2 response was observed in the jejunum of A. galli-infected chickens with increased expression of IL-13 and decreased expression of IFN-γ from day 14 post infection. At the putative time of larvae invasion into the intestinal mucosa (day 7), an increased expression of IFN-γ, IL-10, and TGF-β4 was observed in the spleen. At the putative onset of the innate immune response (day 10), a decreased expression of jejunal IFN-γ and IL-13 was observed. Finally, at the expected period of an adaptive immune response (days 14–21) a general decreased expression of IFN-γ and TGF-β4 in spleen and IFN-γ in jejunum was followed by a decreased expression of IFN-γ and IL-10 at day 21 in caecal tonsils. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect