Molecular characteristics of established trophoblast-derived cell lines
Autor: | Jana Pastuschek, José Martin Murrieta-Coxca, Ruby N. Gutiérrez-Samudio, Diana M. Morales-Prieto, Juliane Sanft, Tanja Groten, Berthold Huppertz, Udo R. Markert, Olivia Nonn, Jasmin Müller |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Placenta Vimentin Biology Cell Line 03 medical and health sciences 0302 clinical medicine Cell Movement Pregnancy microRNA medicine Animals Humans Secretion Cell Proliferation 030219 obstetrics & reproductive medicine Choriocarcinoma Obstetrics and Gynecology Trophoblast medicine.disease Trophoblasts Cell biology MicroRNAs 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Cell culture embryonic structures biology.protein Female Immortalised cell line Intracellular Developmental Biology |
Zdroj: | Placenta. 108:122-133 |
ISSN: | 0143-4004 |
Popis: | Introduction Research on human placental development and function lacks a conclusive in vivo model. To investigate the intracellular molecular mechanisms in trophoblast cells, different cell lines have been established during the last decades. So far, none of these accomplishes all features of primary trophoblast, thus their suitability as well as the transferability of the results has been discussed. The aim of this study is to assess molecular markers and features matching different trophoblast subpopulations in trophoblastic cell lines to provide orientation on their suitability and relevance for distinct research questions. Methods The commonly used trophoblastic cell lines, BeWo, JEG-3, HTR-8/SVneo, AC1-M59, AC1-M32, ACH-3P and Swan71 were selected. qPCR and immunoblotting were used to determine expression of characteristic molecular markers. C14MC, C19MC and miR-371-3 miRNA expression were investigated by real time PCR. Proliferation, migration and network stabilization assays were performed. Hormone secretion was determined by chemiluminescent-immunoassays. DNA profiles were obtained by Short Tandem Repeat (STR)-genotyping. Results Immortalized cell lines differ from choriocarcinoma-derived ones in the expression of HLA-G, E-cadherin, N-cadherin, VE-cadherin, cadherin-11, cytokeratin 7, vimentin, ADAM12 and PRG2. Compared to choriocarcinoma-derived cell lines, expression of C19MC and hormone secretion were almost absent in immortalized cell lines. Conversely, they express C14MC and exhibit higher migration and network stabilization. Discussion The data presented will help justify the use of a cell line to evaluate distinct features of trophoblast biology and pathology. In general, characteristics and markers of choriocarcinoma derived cell lines seem to be more similar to in vivo trophoblast than immortalized cell lines and thus might be regarded as more suitable models. |
Databáze: | OpenAIRE |
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