Cathepsin Y Expression is Up-Regulated in Liver and Spleen of the Rats Growing Under a Low Protein Diet
| Autor: | Keiko Yamafuji, Eri Nakazono, Takayoshi Yamaguchi, Hiroko Tsuda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Pathology Endocrinology Diabetes and Metabolism medicine.medical_treatment Spleen lcsh:TX341-641 Cathepsin L Low-protein diet Internal medicine Casein Gene expression medicine lcsh:RC620-627 Cathepsin Nutrition and Dietetics biology business.industry Skeletal muscle Muscle atrophy lcsh:Nutritional diseases. Deficiency diseases medicine.anatomical_structure Endocrinology biology.protein medicine.symptom business lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrition and Metabolic Insights, Vol 2009, Iss 2, Pp 17-25 (2009) Nutrition and Metabolic Insights, Vol 2 (2009) |
| ISSN: | 1178-6388 |
| Popis: | Background/AimsWe have previously isolated a novel lysosomal cysteine protease, cathepsin Y, which produces a kinin-potenciating octapeptide from rat plasma. The present study aimed to elucidate the influence of protein-restriction on cathepsin Y mRNA expression in tissues of growing rat.MethodsWeaning rats were fed either 20% casein (normal-protein, NP) or 5% casein (low-protein, LP) diet for 12 weeks, from post-weaning period to maturity. Cathepsin Y mRNA expression in tissues was examined using RT-PCR and compared with that of cathepsin L, a general marker of muscle atrophy.ResultsBody, liver and spleen weights and diet intake of LP were significantly lower than those of NP. Cathepsin Y expression in LP was increased in liver (3.70 fold vs. NP) and spleen (1.28 fold) at early periods of protein-restriction, whereas that of cathepsin L was increased in liver and pectoral muscle through all intake periods. However, significant differences were detected in neither cathepsin Y expression in pectoral muscle nor cathepsin L expression in spleen throughout intake periods.ConclusionsA significant up-regulation of cathepsin Y was observed in spleen and liver, suggesting that it may associate with immunodepression in rats under protein-malnutrition during the early post-weaning period. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
http://www.la-press.com/cathepsin-y-expression-is-up-regulated-in-liver-and-spleen-of-the-rats-a1448
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